الفهرس 
INTRODUCTION
Plants MaterialsOnly 14 pages are availabe for public view
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Abstract
Ten wild medicinal plants collected from Sinai, Egypt, were investigated in order to gain insight into its genetic variation based on evidence obtained from karyotype analysis, random amplified polymorphic DNA (RAPD) technique and inter simple sequence repeat (ISSR) technique. The somatic chromosome counts observed as follow: 2n=22 for Citrullus colocynthis,2n=28 for Hyoscyamus boveanus, 2n=20for Phlomis aurea,2n=24for Peganum harmala ,2n=46 for Verbascum sinaiticum ,2n=30 for Origanum syriacum, 2n=44 for Avicennia marina, 2n=24 for Salvadora persica, 2n=16 for Alhagi graecorum and 2n=18 for Anabasis articulata . Karyotype studies showed that the taxa under study have different karyotype formula. However the chromosome type nearly submetacentric (-) is representing in all karyotype formula of all taxa. Biochemical analysis includes protein analysis and two isozymes electrophoresis. In regarding to random amplified polymorphic DNA (RAPD) technique, five primers gave reproducible results with differences in their band numbers. Moreover, the percentage of polymorphism produced by each primer differs from one primer to the other. The percentage of polymorphism generated from RAPD being lowest (6.25 %) for C. colocynthis and Ph. aurea and highest (59.07 %) for H. boveanus, while the polymorphism percentage using ISSR ranged from 6.25% to 56.25% for A. marina and C. colocynthis respectively. The results obtained from karyological features and molecular genetic information in the present study could be used as a part of National Gene Bank strategy for sustainable conservation of endangered and threatened plant genetic resources.
Keywords: Sinai, karyotype,Isozymes, SDS, medicinal plants, RAPD, ISSR.