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العنوان
Biotechnological studies on β-glucosidase from some microorganisms /
المؤلف
Owis, Eman Ali Hassan.
هيئة الاعداد
باحث / ايمان على حسن عويس
مشرف / عبدالدايم أبو الفتوح شريف
مشرف / سامية على هارون
مشرف / نوره الأحمدى على النجار
الموضوع
Industrial microbiology. Biotechnology.
تاريخ النشر
2013.
عدد الصفحات
168 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الأحياء الدقيقة
تاريخ الإجازة
01/01/2013
مكان الإجازة
جامعة المنصورة - كلية العلوم - Botany
الفهرس
Only 14 pages are availabe for public view

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from 189

Abstract

The present work aimed to study the β-glucosidase production and its properties to obtain maximum production by different fungi using different optimization methods under solid state fermentation on rice straw. The fungal strains were isolated from different soils, rice straw and compost samples. The most active fungi in β-glucosidase production under SSF were selected. Plackett-Burman fractional factorial design was used to screen different nutrients and physical factors and choose the most significant factors that affected the production of β-glucosidase. β-glucosidase production was optimized using response surface methodology (Box-Behnken design) in order to obtain the highest production of β-glucosidase. The enzyme was purified by gel filtration method. In addition, physiochemical and kinetic properties of this enzyme were studied.