الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 97 |  |  |
| | | from | 97 | | |
Abstract
Mesenchymal stem cells have been shown to exist in cord blood.
Although MSCs have been described by a subset of surface antigens after
expansion, little is known about the cell surface phenotype of
undifferentiated MSCs. Human umbilical cord blood stem cells able to
differentiate into hepatocytes and fibroplasts in vitro.
The present study is conducted to obtain an HSC-enriched
population (lineage-negative and positive CD34 cells) from the UCB and
characterize their proliferation and differentiation capacities under some
physiological conditions available and easy for application in both basic
and clinical research.
The present study showed human umbilical cord blood stem cell
differentiation; human umbilical cord blood mononuclear cells were
separated by MACs technology. Fibroblast and hepatocyte cell extract
were added in the inducing groups. The expansion and differentiation of
expression of hepatic and MSC markers in differentiated cells was
analyzed by reverse-transcription polymerase chain reaction, and
compared with control.
The present study showed that stem progenitor cells purification
were performed by phenotypic analysis in Flow Cytometry using CD34-
FITC, human kit showed cell purity (76.12±4.02) % (Fig.5). The new
selected CD34+ cells were spherical and remains in its viable state and
forming colonies when growth medium were resuspended in 10% and
changed every 3 days for 10 to 14 days of incubation at 37°C and 5% CO2
incubator (Fig.6) Following treatment with fibroblast cell extract, the majority of CD34+ cell morphology are spherical shape, some cells
behave adherent growth, protruding and spindle cells were seen
(Fig.7).The spherical shape of CD34+ cells treated with fibroblast cell
extract were significantly amplified by adding CD34-, whereas the
cytoplasm of the round cells was enlarged, the spindle-shaped cells had
differentiated into fibroblast-like cells during the time course (Fig.8). The
treatment of the newly selected CD34+ cells with CD34- and hepatocyte
cell lyste , the spherical shaped cells forms regenerative embryonic
bodies differentiated into polygonal cells characteristic to hepatocyte
cells(Fig.9,10). All treated groups expressed optical density of RNA
pattern compared to control.
In conclusion, both of hepatocyte and fibroblast cell homogenate
were sufficient for expansion of UCB CD34+ -cells and co-transplantation
of MSC with UCB CD34+ cells, promoting engraftment of UCB CD34+cells.