الفهرس 
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Abstract
The study was carried out to investigate the mycobacterial strains
isolated from suspected tuberculous like lesion in non-tested tuberculin slaughtered cattle on military abattoir. Specific attention was directed to the tuberculous mycobacteria. The diagnostic evaluation value of different antigens by ELISA test consequently the present study showed the following:
* from 1500 cross breed beef cattle, 60 showed suspected tuberculous like
lesion on PM examination.
* Out of 700 (age less than 2.5 years) slaughtered cattle, 6 (0.85%)
showed tuberculous like lesion.
* Out of 400 slaughtered cattle (aged 2.5 - 3 years), only.20 (5%) showed
suspected tuberculous like lesions.
* Out of 250 slaughtered cattle (aged 3.5 - 4 years), only 20 (8%) showed
suspected tuberculous like lesion.
* Out of 150 slaughtered cattle (aged more than 4 years), only 14 (9.3%) showed suspected tuberculous like lesions.
The bacteriological examination of 60 suspected slaughtered cattle, only 41 gave positive culture.
The biochemical identification of isolated acid fast bacilli revealed that 37 (61.7%) M. bo~s and 4 (6.7%) MOTT. The (4) atypical mycobacteria identified as 2 M. scroffilaceurn and 2 unidentified rapid grower non-chromogenic mycobacteria.
The 37 M. bovis were isolated as following:
* 23 from pulmonary lesion.
* 4 from extra-pulmonary.
* 8 from mixed infection lesion.
* 2 from generalized lesions.
While, the 4 MOTT, 2 from extra-pulmonary lesions and 2 from mixed lesions. The result of ELISA on sera of 60 suspected tuberculous like lesions, at serum dilution 1:80 only 49 (81.7%), 45 (75%) and 46(67.7%) gave positive result with human PPD, bovine PPD and sarkosyl antigens respectively.
The result of ELISA on sera of 41 serum samples from animal which gave positive culture on dilution 1:80,35 (85.4%), 36(87.8%) and 38 (92.7%) gave positive result with human PPD, bovine PPD and sarkosyl antigens respectively.
The result of ELISA on sera of 19 negative culture from suspected
tuberculous lesion of slaughtered animals, 7 (36.8%), 5(26.3%) and 4 (21 .1%) gave positive result with human PPD, bovine PPD and sarkosyl antigens respectively.
The results of sensitivity test of M. bovis to specific anti-tuberculous drugs by proportional method as following:
64.9% to isoniazid, 67.7% to streptomycin, 81.1% to ethambutol, 75.7% to rifampin and 86.5% to thiacetazone.
* The results of sensitivity test of M. scroffilaceum as following:
50% with ethambutol and thiacetazone and rifampin and was resistant to isoniazid and streptornycin.
Finally, the unidentified rapid grower non-chromogenic gave 50%
sensitivity to ethambutol and thiacetazone and not sensitive to isoniazid, streptornycin and rifampin.
from the present study, it can be concluded that:
1. The high percentage of beef cattle showing tuberculous like lesions, which indicated that there is urgent need to tuberculin testing of beef cattle before slaughtering.
2. Using of ELISA test with more specific antigens such as sarkosyl on epiderniological studies of diagnosis of bovine tuberculosis on conjunction with tuberculin test overcomes the problems of the tuberculin test.
3. Finally, the sensitivity test of the isolate mycobacteria showed the high resistant of MOTT to specific anti-tuberculous drugs specially streptornycin, isoniazid and rifampin.