الفهرس 
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Abstract
This work was carried out to survey Salmonella enteritidis
infection in chickens at Kafr El-Sheikh province. The effect of
chemotherapeutic agents on S. enteritidis isolates and
characterization of isolated S. enteritidis were also studied in
addition to the pathogenicity of S. enteritidis isolates.
The results were summarized in the following:
1. Out of 120 examined broiler flocks, 10 were positive for
Salmonella with flock infection rate of 8.3%.
2. Ten isolates were recovered and serotyped which revealed that
6 isolates were S. enteritidis (60%) and 4 isolates were
untyped Salmonellae (40%).
3. Using different selective enriched media: 2 of 30 (6.6%)
samples enriched in selenite F broth, Salmonella isolates were
recovered, 1 of 16 (6.2%) could be recovered using TBGbroth,
and 7 of 74 (9.4%) Salmonella isolates were obtained using
DSE.
4. In vitro sensitivity study showed that cephradine, norfloxacin,
gentamycin, enrofloxacin, doxycycline, ciprofloxacin,
chloramphenicol and oxanilic acid were 100% effective
against all the tested six S. enteritidis isolates. On the other
hand, all of the six isolates of S. enteritidis were resistant to
amoxycillin (100%).
5. SDS-PAGEfor 6 S. enteritidis isolates showed that.all isolates
were of the same clonal type (typical protein makeup).
6. SDS-PAGE revealed that S. enteritidis isolates contained 13
protein bands ranged from 199 to 15 KDa. The most
prominent two bands were at 36 and 32 KDa.
7. Detection of plasmid in S. enteritidis isolates revealed also
appearance of two different plasmid molecular weights 8 and
6 Kbp.
8. In experimental infection, mortality rate among S. enteritidis
isolates varied from 13.8 - 55% with an average of 22.7%.
9. Liver (36.6%) appeared to be colonized with S. enteritidis more
frequently than cecum (20%).
10. Re-isolation of S. enteritidis indicated that the incidence of
recovery of S. enteritidis from the liver was higher than from
cecum (74%vs 65%).
11. Shedding rate reached the peak curve at 7 days postinfection.
After taking cloacal swabs at 1, 2, 3, 7, 14 and 21 days
postinfection, the range of shedding rate was 47.2,47.2,77.7,
100,58.3 and 50%, at the same arranged.