الفهرس 
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Abstract
Dermatophyte infection considers as one of the most important fungal skin affections which infects human and animals. Although this infection is not fatal, however, it has a great effect on the productive capability of the animals and so on the economy, together with its zoonotic transmission. Therefore, the concurrent work was aimed to identify dermatophyte species which infect cattle and horses as the identification of the species and strain of dermatophyte can play an effective role in control of disease outbreaks. In addition, the current methods of identification are based on cultural and microscopic methods, and these often involving weeks before a positive identification are made. So, a rapid molecular diagnostic method would therefore be an important laboratory technique.
This work includes examination of hairs and skin scrapings that were collected from horses (55 samples) and cattle (60 samples) in order to isolate and identify the causative dermatophyte species, aiming to find a diagnostic method that give sensitive and specific results for dermatophytes within hours. These samples were subjected to direct microscopic examination, cultivation on Sabouraud’s dextrose agar, microscopical examination of colonies by using Lactophenol cotton blue stain, followed by some biochemical and hair perforation test. The mycological examination of samples collected from 55 horses
revealed isolation of 18 (32.73%) positive culture out of 55 samples were identified as T.verrucosum species. While in cattle, there was 42 (70 %) positive culture out of 60 samples were identified as T. verrucosum species.
Among the positive samples only 6 samples were subjected to molecular based techniques for dermatophyte diagnosis. Directly extracted DNA samples were subjected to PCR reaction using a primer pair specific to chitin synthase 1 gene, Primer Trich302-5for and Trich302-5rev and nonspecific simple repetitive oligonucleotide (GACA)4 . The results showed that the usage of the third primer was able to differentiate between the isolated species and detect T. verrucosum species according to different band size.
from the previous obtained results (conventional methods and PCR) we can conclude that, the molecular based diagnosis of dermatophytes is more sensitive, specific and give a very rapid result comparing with the traditional methods.
Dermatophyte infection considers as one of the most important fungal skin affections which infects human and animals. Although this infection is not fatal, however, it has a great effect on the productive capability of the animals and so on the economy, together with its zoonotic transmission. Therefore, the concurrent work was aimed to identify dermatophyte species which infect cattle and horses as the identification of the species and strain of dermatophyte can play an effective role in control of disease outbreaks. In addition, the current methods of identification are based on cultural and microscopic methods, and these often involving weeks before a positive identification are made. So, a rapid molecular diagnostic method would therefore be an important laboratory technique.
This work includes examination of hairs and skin scrapings that were collected from horses (55 samples) and cattle (60 samples) in order to isolate and identify the causative dermatophyte species, aiming to find a diagnostic method that give sensitive and specific results for dermatophytes within hours. These samples were subjected to direct microscopic examination, cultivation on Sabouraud’s dextrose agar, microscopical examination of colonies by using Lactophenol cotton blue stain, followed by some biochemical and hair perforation test. The mycological examination of samples collected from 55 horses
revealed isolation of 18 (32.73%) positive culture out of 55 samples were identified as T.verrucosum species. While in cattle, there was 42 (70 %) positive culture out of 60 samples were identified as T. verrucosum species.
Among the positive samples only 6 samples were subjected to molecular based techniques for dermatophyte diagnosis. Directly extracted DNA samples were subjected to PCR reaction using a primer pair specific to chitin synthase 1 gene, Primer Trich302-5for and Trich302-5rev and nonspecific simple repetitive oligonucleotide (GACA)4 . The results showed that the usage of the third primer was able to differentiate between the isolated species and detect T. verrucosum species according to different band size.
from the previous obtained results (conventional methods and PCR) we can conclude that, the molecular based diagnosis of dermatophytes is more sensitive, specific and give a very rapid result comparing with the traditional methods.