الفهرس 
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Abstract
This work was carried out to study the occurrence of Listeria species in different food, animal and human samples. A total of 1386 samples were collected from Cairo and Giza governorates (553 different food samples, 504 fecal samples and 257 vaginal swabs from different animals and 72 human samples). The occurrence of Listeria spp. in the examined food samples was 12.10%, 0.62%, 2.94%, zero and 1.89%, and the occurrence of Listeria monocytogenes in the examined food samples was2.55%, 0.62% and 0.94% in the examined milk samples from dairy shops, milk samples from farms and eggs, respectively.On the other hand failed to isolate L.monocytogenesfrom cheese and meat products.The occurrence of Listeria spp. in the examined fecal samples of sheep and goat in this study was2.33% and 1.45%, respectively and failed to isolate Listeria from vaginal swabs of different animals. The occurrence of Listeria spp. in stool samples of neonates and children, immunosuppressed persons and farm workers was 4.35%, 5.56% and zero, respectively. The isolated Listeria spp. wasL.monocytogenes (4.35%) from neonates and children andL.ivanovii (5.56%) from immunosuppressed persons. By PCR only six samples were positivefor L.monocytogenes, Four milk samples (2.55%) from dairy shops were positive, one milk sample (0.62%) sample from farms and one stool sample (4.35%) of neonates and children, but by CHROMagar seven samples were positivefor L.monocytogenes those detected by PCR in addition to another one milk sample from dairy shops and by conventional method eight samples were positiveforL.monocytogenes those detected by PCR beside one(2.50%) from hen’s egg shell and one (0.58%)from fecal sample of sheep. PCR primer targeting the hemolysin (hlyA) gene was used for confirmation the isolation ofL.monocytogenes. The sensitivity and reliability of PCRwas comparablewith CHROMagar and conventional methods.
As knowledge of molecular and applied biology of L.monocytogenes increases, progress can be made in the prevention and control of human infection.