الفهرس 
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Abstract
I Isolation and identifcation of P. multocida and P. haemolytica organisms were carred out from nasopharyngeal swabs and pneumonic lungs of 207 slaughtered calves at 5 abattoirs in Sharkia province.
a - 6 P. multocida strains from nasopharynx in percentage (2.89%) and 7 strains from pneumonic lungs (3.33%) .
* - Serotyping of the obtained isolates using indirect
haemagglutination test revealed that (13) P. multocida strains were belonged to the capsular group ”B” and using gell diffusion precipitation test revealed that all of them belonged to somatic type 2 (B: 2).
b - 9 P. haemolytica strains were isolated from nasopharynx of slaughtered calves (4.35%) and 22 from pneumonic lungs of slaughtered calves (10.62%).
The biotyping tests revealed that the ”31” P. haemolytica isolates were (24) biotype ”A” 77.45% , (7) biotype ”T” 22.55% .
c- P. multocida was isolated more frequent in buffaloe calves (5.55%) while in cow calves it was 0.19% from nasopharyngeal swabs P. haemolytica was more frquently isolated (5.56%) from buffaloe calves while it was (3.92%) in cow calves from nasopharyngeal swabs, but the rate of isolation from pneumonic lung lesions were 11.11% and 9.26% in cow and buffaloe calves.
II - Three pasteurella vaccines were prepared against P. multocida and P. haemolytica .
a - Trials of vaccination were carried out on 4 groups of calves (16 non vaccinated male native breed cow calves) . They were divided into 4 groups, 4 animals in each group.
The first group received monovalent P. multocida oil adjuvant vacccine.
The second group received bivalent P. haemolytica formalized vaccine.
The third group received a combined P. mulltocida and P. haemolytica oil adjuvant vaccine.
The fourth group were kept as control ( non vacciinted ) calves .
b- Sera samples of vaccinated and control groups were collected at different intervals up to 150 days post initial vaccination . One portion of these samples individually subjected to indirect haemagglutination test (II-IA). The other portion of vaccinated groups were pooled and used for application of mouse protection test .
III The active mouse vaccination challenge inoculation system was used to evaluate the efficacy of the prepared vaccines and revealed that 3.53 log protection could be acheived by P. multocida oil adjuvant vaccine 3.95 log and 3.57 log protection by combined oil adjuvant vaccine and 3.64 log protection by P. haemolytica formalized vaccine.
A Combined pasteurella oil adjuvant vaccine, P. multocida oil adjuvant vaccine and P. haemolytica formlized vaccine can confer , stable high percentage of protection against pasteurellosis for at least the fattening period of calves.
1 - Pasteurella multocida was isolated from nasopharyngeal swabs of cow and buffaloe calve in a percentage of 2.89% while the percentage of isolation from pneumonic lungs lesions was 3.38% .
* - The isolated strains were typed biochemically, serologically and they belonged to B: 2 serotypes.
2 - Pasteurella haemolytica was isolated from nasopharyngeal swabs in a percentage of 4.35% and in pneumonic lung lesions in a percentage of 10.62% .
* - The isolated strains were biotyped and they belonged to type ”A” (77.45%) and Type ”T” (22.55%) .
3 - A combined vaccine prepared from P. multocida and P. haemolytica isolated strains was proved effective was evaluated by passive mouse protection test , active mouse protection test and indirect haemagglutination test .
a - The highest antibody titre as measured by indirect haemogglutination test was 5120 started from 2 nd and 3rd month and persist up to 5 months of fettening period ( the end of the experiment ).
b - Passive mouse protection test detects protection in prepared vaccine at 3 weeks after initial vaccination.
c - The log protection afforded by the combined vaccine was (3.95) and (3.57) by active mouse protection test in comparison with (3.53) for monovalent Pasteurella multocida vaccine and (3.64) for P. haemolytica vaccine .
4 - Combined pasteurella oil adjuvant vaccine can confer a stable , high persisting protection against pasteurellois for at lest the fattening period of calves.
5 - As evaluation of the used vaccines in the study by IHA test, passive mouse protection test and active mouse protection test , the combined vaccine prepared from P. multocida and P. haemolytic isolated strains was proved to be effective than monovalent P. multocida and P. haemeolytica vacccines.