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العنوان
Incidence and public health importance of food Poisoning causative organisms in milk.and some dairy products in kaliobia governorate /
المؤلف
El-Bagoury, Abd EL-Rahman Mahmoud Abd El- Rahman.
هيئة الاعداد
باحث / عبد الرحمن محموود عبد الرحمن الباجورى
مشرف / عبد الوهاب مرسى فهمى
مناقش / سعد محمود سعد
مناقش / عبد الوهاب مرسى فهمى
الموضوع
Dairy products.
تاريخ النشر
1991.
عدد الصفحات
145 p. ;
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Food Animals
تاريخ الإجازة
1/1/1991
مكان الإجازة
جامعة بنها - كلية الطب البيطري - control food
الفهرس
Only 14 pages are availabe for public view

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from 159

Abstract

Two hundred random samples,fifty each, of raw market milk, fresh cream, Domiati cheese and ice-cream procured from different localities in Kaliobia governorate were bacteriolo-gically examined for incidence of food poisoning causative organisms.
Bacteriological examination of collected samples of raw market milk,fresh cream,Domiati cheese and ice-cream revealed that,Staph.aureus could be detected in 50%, 44%, 26% and.22%, respectively,with mean counts of 1.10 x 104± 0.23 x 104, 4.70 x 104 ± 1.90 x 104 , 5.9 x 103 ± 2.7 x 103 and 1.80 x 103± 0.27 x 103,respectively.
All strong coagulase positive Staph.aureus strains (4+ score) produced thermonuclease, while 89.47% , 51.85% and 40% of coagulase positive strains (3+ , 2+ & 1+ scores),respecti-vely produced thermonuclease.
Twenty three Staph.aureus strains out of 95 coagulase positive strains (4+ score) isolated from the different products were tested for enterotoxin production.
Enterotoxins were producted by 71.43% , 100% , 100% and 80% of the tested strains isolated from raw market milk,fresh cream,Domiati cheese and ice-cream samples,respectively. En-terotoxins belonging to A , B , C , D , AC , BC , CD and ACD types could be detected.
Salmonella muenchen could be isolated from only one
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sample of examined market raw milk (2%). Serological typing proved that the isolated salmonella strain has the antigenic structure of 6,8 : d: 1,2 (Sal.muenchen).
Salmonellae failed detection in the other products.
Coliforms could be detected in 100%,100%,38% and 94% of examined raw milk,fresh cream,Domiati cheese and ice-cream samples,respectively.The mean counts (MPN/100 ml. or g.) were 4.53 x 1010 ± 1.92 x 1010, 6.1 x 1012 ± 1.6 x 1012, 2.1 x 105 ± 1.3 x 105 and 1.44 x 1010 ± 0.68 x 1010 , respectively.
Escherichia coli could be isolated from 16%, 18%, 15.79% and 17.02% of the positive samples for raw milk, fresh cream, Domiati cheese and ice-cream, respectively. While
Enterobacter aerogenes, Ent.gergovia, Ent.cloacae, Ent.agglumerans and Ent.intermediate could be isolated in varying percentages from 0 - 46% . Citrobacter freundii, Cit.amaloniticus and Cit.diversus could be isolated at varying percentages (2.08% to 29.17%). While Klebsiella oxytoca, K.ozaenae, K.rhinoscleromatis and K.pneumoniae could be isolated at varying percentages (2% to 31.25%).
Serological typing of isolated E.coli strains from examined products revealed the identification of 5 serotypes
belonging to : OKB, 0111 K71 K59 B5 111 K58 B4 126 71 16’ 0125
K70 B15 and 0124 K72 B17.
Clostridium perfringens could be isolated from 48%,26%, 68% and 44% of the examined raw market milk , fresh cream , Domiati cheese and ice-cream samples , respectively , with
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average counts of 4.45 , 789 , 215 and 3.64 / ml. or g., respectively.
All of the 130 strains of Cl.perfringens isolated from examined samples proved to be enteroxin A producers.
Bacillus cereus could be isolated from 6% , 0% , 18% and 16% with an average count of 9100 ,0 ,400 and 820 / ml. or g. of examined samples,respectively.
Enterococcal count/ml. or g. of raw market milk, fresh
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cream , Domiati cheese and ice-cream samples were 4.30 x 10±
2.40 x 104,2.30 x 104± 0.55 x 104, 9.74 x 104 5.12 x ro4and
2.60 x 104 ± 1.20 x 104,respectively.Strept.faecalis could be isolated from 52%,62%,48% and 66% of the examined products, respectively.
The public health importance of isolated organisms as well as suggested sanitary control measures for improving the quality of milk and its products and to safeguard consumers from being infected has been discussed.