الفهرس 
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Abstract
The present work of this study was carried out during the breeding seasons early breeding season (spring) and late breeding season (autumn). At El-Abbasa Fish Hatchnary, Sharkia govern mate. A total number of 90 Carp (Cyprinus carpio L.) (60 males and 30 females) The purpose of this study to make complete report for milt of carp fish and uses other substance (hormones + DOPA antagonists) in spite of pituitary extract and extender for milt cryopreservation were used .
In current study; males were divided into 6 groups
1-The first group were treated by pituitary gland extract and anaesthetized.
2-The second grOup treated by pituitary gland extracts and not anaesthetized.
3-The third group treated was anaesthetized and treated by GnRH.
4-The fourth group treated was anaesthetized and treated by GnRH and Dopamine antagonist
5- The fifth group was anaesthetized and not treated and was used again at the late breeding season, autumn for induced propagation .The fish was anaesthetized and injected with LHRH and dopamine antagonist
6-The sixth group was neither treated or anaesthetized.
The pituitary gland were prepared from a mature carp fish, and then injected interaprotenial in a dose between 0.5 — 2.5 / kg b.w dissolved in saline not more than 5 cm for males but females the dose divided into two doses the first 90% and the second (10 5) after 12 hour, a group were treated by GnRH in dose 15ug/kg b.w, the other group were treated by GnRH + DOPA antagonists, all fishes were anesthetized by Ms222 or thiopental and then milt sample were examined.
y in fertilizing solution ( 2.8 ± 0.12
±0.10
) , (1.4 ± 0.10 ) ,
( 0.9
± 0.1
25 )2(
0.4 ±0.19 ),(0.1
±0.1
)(O.
n in mass motility in fertilizing soluti n isied water (0.00 ± 0.0) , (0.00 + 0.0).
100
SUMMARY
1-There was no significant diffe extract nor treated by GnRH antagonists.
2-The effect of seasons (Spring Carp fishes showed a highly signi autumn seasons in the milt volin the live sperm (97.60 ± 0.51 v significant difference was obsery 3.50 5 0.22 kg), the sperm motilit the sperm count (2.46 ± 0.81 vs 2.
3-) Effect of different solutions spermatozoa
a- There was no motility in
ence for milt treated either by pituitary lone or treated by GnRH + DOPA
nd Autumn) on the milt characters If scant (P < 0.01) increase in spring th
e (5.60 ± 0.40 vs 1.80 ± 0.25 ml) d
93.80 ± 0.58 %). However, a no d in the body weight (4.40 ± 0.87 (80.00 ± 3.16 vs 76.00 ± 2.91 %) a d 0 ± 0:53 x1012/m1) respectively .
in initiation of mass activity of fre h
aline solution
b- In decholrinated wat significant variation in (1.70 + solution (3.5 ± 0.22), (3.2 ± 0.12) significant increase in mass activi ( 2.4 ± 0.10 ) , (1.9 ±0.10 ) ( 1.4 than dechlornisied water (0.80 ± 0 ± 0.00 ) , in 240 , 270 no variati • ( 0.3 ± 0.00 ) (0.00 ± 0.0) dechlo
r (30, 60 second ) there was non 0.12), ( 1.30 ± 0.25) and fertiliz. g , in ( 90 , 120 , 150, 180, 210, secon )
0
3- There was significant increase n mass activity of fresh semen ( 3.40 ± 0.29 ) than frozen semen ( 2.4 ± 0.19 0 .there was also signific t increase in individual motility of liesh semen ( 84.0 + 0.87 ) than frozen 9
55.0 ± 2.24 ) .Dead % of fresh s men was significantly increase in fre .h • semen % ( 6.60 ±0.21 ) than frozen semen ( 24.0 ± 2.22) .
4-Effect of temperature and time ii mass motility on thawing straw:-
the best post thaw motility was obtained after thawing between bo h hands (3.0 ± 0.00) for 30 seconds -id at 30 OC for 15 , 40 °C.
101 SUMMARY
5- Electronmicrogjaph milt of Common carp show normal structure of milt with a rounded head, a midpiece and a tail region, but no acrosom .The head is spherical and the nucleus occupy all the head region .The midpiece is cylindrical .It contains two mitochondria process in the tail region show the flagella which isqlomposed of two central microtubules and nine peripheral microtubules.
6- Electronmicrograph of Common. Carp testicles Section in the inner part of lobule filled up with spermatozoa. Observed in mature sexual lobular type; the lobules were irregular shape in variable size. All
•
developmdntal stages were observed inside the lobules, at the edge of the
lobule and within individual cysts the spermatocytes (I) are slightly bigger than the spermatocytes (II) and have dense and less rounded nuclei The spermatids even smaller than the spermatocytes II have very dense, rounded nuclei, in the inner part of the lobules spermatozoa are packed and fill the lobular lumen.
7- Transverse section through testis of Common carp before treatment general aspect of lobule organization, partial view of section showing narrow seminiferous tubules with a few cellular lining, little numbers of spermatozoa and thin tunica albuginia also showing the all development stages inside the lobules, at edges of lobules within individual cysts .The spermatocytes I are slightly bigger than the spermatocytes H have very dense. Rounded nucleus .In the inner part of the lobules spermatozoa are packed and fill the lobular lumen.
8-Fertilization was followed up by light microscope in its all division periods.
9-Fertilization were made by using frozen milt and followed the stages of development.