الفهرس 
REVIEWOnly 14 pages are availabe for public view
 |  | from | 181 |  |  |
| | | from | 181 | | |
Abstract
The present study was designed to investigate biochemical and cytological changes of peritoneal fluid results from different types of peritonitis as well as evaluating the associated hematological, biochemical changes of the blood and histopathological alterations in organs caused by peritonitis. In this study, 90 male Albino rats (180-250 gm body weight) were used. Rats were randomly allocated into five groups (18 rats/group) and housed in separate cages as following:Group (C): contains 18 rats which they were used as control as treated with sterial saline. Group (TGL): 18 rats which they were injected with thioglycolatte medium (low dose 1ml/100 gm body weight) intraperitoneal. Group (TGH): 18 rats treated with thioglycolate medium (high dose 2ml/100 gm body weight) which injected intraperitoneal. Group (ECL): 18 rats which they were infected with E.coli (low dose 0.5× 108 CFU) by intraperitoneal injection. Group (ECH) : 18 rats which experimentally infected with E.coli (high dose 1× 108 CFU) by intraperitoneal injection. The experiment was continued for 2 weeks, during which all groups were observed and collection of samples from 6 rats from each group were performed at 1 day, 1 week and 2 weeks after induction of peritonitis. Samples included peritoneal fluid for biochemical and cytological analysis, blood for hematological and biochemical analysis and tissue specimens for histopathological examination. Peritoneal fluid analysis included biochemical and cytological analysis, while hematological analysis includes RBCS count, PCV, Hb concentration, RBCS indices (MCV, MCH and MCHC), WBCS count, differential leukocytes count and blood platelets. Serum biochemical parameters includes total protein, albumin, globulin, AST and ALT activities, total bilirubin, direct and indirect bilirubin, cholesterol, triglycerides, sodium, potassium, calcium and phosphorus. Specimens from liver and peritoneum for histopathological examination. The following results were observed: 1. Peritoneal fluid analysis: Chemical and bacterial peritonitis models showed significant changes in chemical and cytological examination of peritoneal fluid. Concerning to chemical examination of peritoneal fluid, rats were injected with low and high doses of thioglycollate medium and E. coli showed significant increases in total protein concentration, SPG and cholesterol levels at all times after induction of peritonitis compared to the control group. TGL and TGH groups showed significant increases in triglycerides level at 1 day after induction of peritonitis compared to the control group. While ECL and ECH groups showed non-significant changes in triglycerides level at all times after induction of peritonitis compared to the control group. Regarding to cytological examination of peritoneal fluid, rats were injected with low and high doses of thioglycollate medium and E. coli showed significant increases in TNCC, RBCS, monocytes, neutrophils and mesothelial cells counts at all times after induction of peritonitis compared to the control group. Chemical peritonitis model showed significant increases mainly in mesothelial cells compared to bacterial peritonitis model. On the other hand, bacterial peritonitis model showed significant increases mainly in neutrophils compared to chemical peritonitis. Both models showed significant increases in lymphocytes count at 1 day after induction of peritonitis compared to the control group. 2. Hematological changes: a- The erythrograme results: Rats injected with low and high doses of thioglycolatte medium and E. coli showed non-significant changes in RBCS, Hb, PCV, MCV, MCH and MCHC at 1 day, 1 week and 2 weeks after induction of peritonitis when compared with the control group. Concerning to blood platelets results, rats injected with high doses of thioglycolatte medium and E. coli showed significant decreases in blood platelets at 1 day after induction of peritonitis compared to the control group. b- The leukograme results: The evaluation of leukogram revealed leukocytosis with significant increases in granulocytes counts in rats injected with low and high doses of thioglycolatte medium and E. coli at 1 day after induction of peritonitis compared to the control group and to 1 week and 2 weeks after induction of peritonitis. While TGL, TGH, ECL and ECH groups showed non-significant changes in monocytes count at 1 day, 1week and 2 weeks after induction of peritonitis compared to the control group. TGL and TGH groups showed non-significant changes in lymphocytes count at all times after induction of peritonitis compared to the control group. While ECL and ECH groups showed significant increases in lymphocytes count at 1 day after induction of peritonitis compared to the control group and to 1 week and 2 weeks after induction of peritonitis. 3. Serum biochemical changes: Concerning to serum biochemical results, rats were injected with low and high doses of thioglycolatte medium showed significant increases in total protein and globulin at 1 week and 2 weeks after induction of peritonitis compared to the control group. Moreover rats injected with low and high doses of E. coli showed significant decreases in total protein and globulin at 1 day after induction of peritonitis compared to the control group. TGL and TGH groups showed significant decreases in albumin level at 1 week and 2 weeks after induction of peritonitis compared to the control group. While ECL and ECH groups showed non-significant changes in albumin level at all times after induction of peritonitis compared to the control group. Chemical and bacterial peritonitis models showed significant increases in ALT and AST activities at all times after induction of peritonitis compared to the control group. While both models showed non-significant changes in total bilirubin, direct and indirect bilirubin and potassium at all times after induction of peritonitis compared to the control group. Thioglycolatte-induced peritonitis model showed non-significant changes in cholesterol and calcium at all times after induction of peritonitis compared to the control group. While E. coli-induced peritonitis model showed hypocholesteremia and hypercalcimeia at different times after induction of peritonitis compared to the control group. Both models showed significant increases in triglycerides level at different times after induction of peritonitis compared to the control group. Also chemical and bacterial peritonitis models showed hyponatermia and hyperphosphatemia at different times after induction of peritonitis compared to the control group.