الفهرس 
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Abstract
The current study was planned to evaluate the possible therapeutic role of bone marrow-derived MSCs (BM-MSCs) and adipose-derived MSCs (AT-MSCs) in management of liver fibrosis induced in albino rats. The mesenchymal stem cells were harvested from femoral bone marrow and from both the omentum and inguinal fat pad of female rats. The isolated BM-MSCs and AT-MSCs were then characterized via their morphological appearance, multilineage potential and PCR detection of CD29, CD44, CD106, CD14, CD34 and CD45 surface markers. The present study was conducted on a total number of 50 adult female albino rats classified into five experimental groups (10 rats/ group). The first was healthy control group (Negative control group). The groups from the second to the fifth were injected intraperitoneally (i.p) with thioacetamide in a dose of 100 mg/kg b.wt three times weekly for 6 weeks to induce liver fibrosis. The second group was left untreated and served as positive control group (TAA). The third group was injected with 0.5 ml culture media alone into the tail vein (i.v). The fourth group was injected with a single dose (0.5 ml) of culture media containing 3x106 BM-MSCs intravenously. The fifth group was injected with a single dose (0.5 ml) of culture media containing 3 x 106 AT-MSCs intravenously. The therapeutic potential of bone marrow derived mesenchymal stem cells and adipose-derived mesenchymal stem cells in management of experimentally liver fibrosis induced in rats was evaluated. This was achieved by assessing relevant biomarkers including; serum levels AST, ALT, and albumin and plasma ammonia level. In addition, levels of serum fibronectin (FN), plasma fibrinogen (FBG), hepatic transforming growth factor (TGF- β1), hepatic hepatocyte growth factor (HGF), and epidermal growth factor (EGF) were estimated using ELISA technique. Besides, the histopathological investigations of the liver tissue sections were performed. The results revealed that the isolated BM-MSCs and AT-MSCs proved their MSCs identity via their typical morphological appearance, multilineage potential which was confirmed via in vitro differentiation to adipocytes, chondrocytes and osteocytes as well as expression of MSCs surface markers; as the BM-MSCs showed positive expression for CD29, CD44 and CD106 beside the negative expression for CD14, CD34 and CD45. While, AT-MSCs shared the same results except for the positive expression of CD45. Moreover, the engraftment of PKH-stained undifferentiated bone marrow or adipose tissue derived mesenchymal stem cells in the liver of thioacetamide administered rats emphasized that the systemically delivered single dose of undifferentiated MSCs was able to home into the thioacetamide injured liver of rats. Furthermore, the biochemical results indicated that the MSCs injection have improved liver fibrosis through the significant decrease of serum AST, ALT, and plasma ammonia levels beside a significant decrease in serum fibronectin and TGF-β levels in addition to the restoration of serum albumin and plasma fibrinogen levels compared with the untreated positive control group. The histopathological investigations of liver tissue section supported the biochemical results as indicated by the significant improvements in the hostological feature of liver tissue following the MSCs infusion as compared to the untreated positive control group.