الفهرس 
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Abstract
This study aimed to : Stem cell transplantation is a promising treatment for liver fibrosis. Mesenchymal stem cells can reduce liver fibrosis by different mechanisms. Splenectomy may also reduce liver fibrosis but its effect on stem cell transplantation was not studied. Aim of the work: To assess the effect of human adipose tissue-derived mesenchymal stem cell (hASC) transplantation and splenectomy on immunocompetent rats. Subjects and methods: Liver cirrhosis was induced in 130 Sprague-Dawley rats with thioacetamide 200mg/kg IP twice a week for 2 months. Eighty rats survived and were divided into 4 groups: control group; 10 rats were sacrificed 1 month after induction of liver cirrhosis, splenectomy group; 50 rats in which splenectomy was performed and the surviving rats (10) were sacrificed 1 month later, stem cell group; 10 rats received 1x106 hASC in 1 ml into the portal vein and were sacrificed 1 month later and stem cell with splenectomy group; 10 rats received portal vein stem cell infusion with splenectomy. Nine rats in this group died and the group was stopped. Blood samples were taken for CBC, ALT, AST and albumin. Liver tissue was stained with hematoxylin and eosin for scoring of liver fibrosis and with masson trichrome for image analysis with imageJ program. Results: Fibrosis score and image analysis were significantly lower while serum albumin was significantly higher in the splenectomy group compared to the control group. Fibrosis score and image analysis were significantly lower while serum albumin was significantly higher in the stem cell group compared to the control group. Serum albumin was significantly higher and both ALT and AST were significantly lower in the splenectomy group compared to the stem cell group. Fibrosis score was significantly lower in the stem cell group compared to the splenectomy group. Conclusion: Both stem cells and splenectomy could reduce liver fibrosis and increase serum albumin in rats with liver cirrhosis. The effect of stem cells was better than splenectomy on fibrosis score while the effect of splenectomy was better on serum albumin, ALT and AST.