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العنوان
Some bacterial foodborne pathogens in cattle, camel and sheep carcasses /
المؤلف
Sabike, Islam Ibrahim Ahmed.
هيئة الاعداد
باحث / اسلام ابراهيم أحمد سابق
مشرف / سعد محمود سعد
مناقش / أبو بكر مصطفى إدريس
مناقش / محمد أحمد حسن
الموضوع
Cattle diseases. Meat analysis. Meat bacteriology.
تاريخ النشر
2011.
عدد الصفحات
151 p. ;
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Food Animals
تاريخ الإجازة
1/1/2011
مكان الإجازة
جامعة بنها - كلية الطب البيطري - مراقبة الأغذية
الفهرس
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Abstract

A total of 150 random swab samples collected from slaughtered cattle, camel and sheep carcasses .The swab samples were taken from two areas represented by rump and brisket (25 of each), the swab samples were examined for total enterobacteriaceae count, total coliform count, total staphylococci count,E. coli, salmonella and S. aureus. The obtained results indicated that the mean values of total enterobacteriaceae count in the examined swab samples of sheep, cattle and camel were 2.54×103 ± 0.44×103/ cm2 & 2.25×103 ± 0.37× 103/cm2 , 1.33×103 ± 1.18×103 & 0.50×103 ± 0.30× 103 and 0.57×103 ± 0.26×103 & 0.26×103 ± 0.10×103 in rump and brisket ,respectively.
Results associated with the examined samples were significant at (P ≤ 0.05) as a result of their bacterial profiles. Furthermore incidence of enteric bacteria outlined in table (4) revealed that the incidence of Citrobacter freundii from rump was isolated from 4% of examined camel samples, but not isolated from sheep and cattle samples .while mean, for brisket samples, Citrobacter spp. was 4% of the examined sheep and camel samples. Moreover, the incidence of Enterobacter aerogenes, Enterobacter agglomerans , Enterobacter cloacae and Enterobacter hafniae were isolated from rump and brisket of sheep, cattle and camel at rate of 8% & 4%; 0% &8% and 20% &12%, respectively, from the examined samples. While, Klebsiella ozaenae and Klebsiella pneumonae were isolated from rump and brisket of sheep, cattle and camel at rate of 8% & 4%; 0% & 4% and 4% & 8% from the examined samples, respectively. Lastly, Proteus mirabilis, Proteus rettgri and Proteus vulgaris were isolated from rump and brisket of sheep, cattle and camel at rate of 8% &8%; 20% &0% and 0% &0% from the examined samples, respectively. Concerning the total coliform count the mean values of examined swab samples of sheep, cattle and camel were 2.97×103±.51×103/cm2 & 2.51×103±.50×103/cm2 , 0.85×103± 0.67×103& 0.97×103± 0.66×103,and 0.28× 103±0.75×103 & 0.48×103±0.11×103 in rump and brisket, respectively. Results associated with the examined samples were significant either (P ≤ 0.01) or (P ≤ 0.05) as a result of their bacterial profiles. Concerning the mean values of total staphylococcus count in the examined swab samples of sheep, cattle and camel were 1.28103± 0.39×103/cm2 &3.17×103± 1.09×103/ cm2, 0.31×103 ±0.10×103 &0.46×103 ± 0.18×103and 0. 31×103 ± 0.16×103 &0.34×103± 0.10 × 103 in rump and brisket, respectively. Results associated with the examined samples were significant either (P ≤ 0.01) or (P ≤ 0.05) as a result of their bacterial profiles.
On the other hand, the incidence of E. coli declared that the total isolates of E. coli in rump and brisket of sheep, cattle and camel were (16%) and (8%) ,(8%) and (0%) ,and (16%) and(8%) ,from the examined swab samples, respectively.
Furthermore, the isolated E.coli were serologically identified as enteropathogenic E. coli O55:K59, enterohaemorrhagic E. Coli O26:K60, enteropathogenic E. coli O86:K61, enteroinvasive E. Coli O124:K72, enterohaemorrhagic E. Coli O111:K58, enterotoxigenic E. coli O128:K67 and enteropathogenic E. coli O119:K69 in the different examined sites of slaughtered food animals with different percentages. The predominant strain of E. coli was E. coli O124:K72, whose incidence was4 isolates (16%) from the examined swab samples.
On the other hand, the incidence of salmonella declared that the total isolates of salmonellae in rump and brisket of sheep, cattle and camel were (0%) and (12%), (8%) and (0%) ,and (0%) and (0%) , from the examined swab samples, respectively.
Moreover, the isolated Salmonella were serologically identified as S. Typhimurium and S. Enteritidis from the examined slaughtered food animal’s carcasses. The predominant strains of salmonella were S. Typhimurium whose incidence was 3 isolates (12%), then S. Enteritidis whose incidence was 2 isolates (8%), of a total twenty five examined swab samples.
Furthermore, the incidence of Gram positive cocci declared that the total isolates of Staphylococcus aureus ,S. epidermidis and micrococci in sheep were (8%) ,(4%) and (0%) for rump site, while for brisket were (8%) , (0%) and (8%) of examined swab samples, respectively. On the other hand, in cattle (4%) was recorded for incidence of S. aureus and S. epidermidis and (8%) for incidence of micrococci in rump site, while for brisket were (0%), (8%) and (12%) of examined swab samples, respectively. The incidence of S. aureus, S. epidermidis and micrococci in camel were (4%), (0%) and (4%) for rump site, while (4%), (0%) and (12%) for brisket of a total twenty five examined swab samples, respectively.
The public health significance of isolated microorganisms and the possible sources of contamination of carcasses with these organisms as well as suggestive hygienic measures to improve the quality of such items were discussed.