الفهرس | Only 14 pages are availabe for public view |
Abstract This study was under taken to show the effect of different doses of gamma radiation (1, 2, 3 and 5KGy) on the metabolism of some seeds born fungi isolated from maize and wheat seeds collected from different local markets from El-Gharbia to control growth of more common fungi during storage. Nine fungi were isolated from maize and wheat seeds. The most common species were Aspergillus terricola and Aspergillus fumigatus. Gamma ray has no effect on maize and wheat seeds germination. Gamma radiation leads to several mutagenic change which affected on germination, viability and germ tube length of Aspergillus terricola and Aspergillus fumigatus spores which decreased by increasing gamma rays. Also, gamma irradiation affected on the dry biomass and radial growth in both fungi. The content of total proteins, carbohydrates and lipids decreased with increasing the irradiation doses. Also, enzymes activities of Aspergillus terricola and Aspergillus fumigatus were affected by gamma irradiation. α- amylase activity decreased when gamma irradiation doses increased whereas, cellulase and protease activities were stimulated. Gamma radiation also affected on free amino acids contents. Gamma irradiation stimulated the production of organic acids such as oxalic and citric acids, in the growth medium of Aspergillus terricola and Aspergillus fumigatus as secondary metabolites. Also, SDS-PAGE electrophoresis gave two new bands under the influence of gamma irradiation. |