الفهرس | Only 14 pages are availabe for public view |
Abstract Aim of Work Poly (Af mRNA from S. mansoni adult worms was used to construct eDNA expression library in lambda-gtll expression vector. Protective antiserum from a rabbit immunized with irradiated cercariae was used to irnmunoscreen the eDNA library. Several clones expressing antigens recognized by protective antisera had been isolated. Our goal is to identify one of the schistosome antigens recognized by this protective antiserum; and characterize its ability to induce protection against schistosomiasis. Characterization will contain: 1- Nucleic acid determination and the deduction of the amino acid sequence. 2- Comparison of the deduced protein sequence \>.’ith otht.:r protem sequences in gene data base. 3- Southern blotting to determine gene copy number. 4- Northern blotting to evaluate mRNA expression. 5- Evaluation of the recombinant protein as a protectivC |