الفهرس 
introduction & review of litratureOnly 14 pages are availabe for public view
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Abstract
Schistosomiasis is debilitating, and sometimes deadly,
parasitic infection that affects hundreds of millions of people
living in tropical and subtropical countries. Chemotherapy,
using effective drug, does not give protection against
reinfection, and in the same time the parasite resistance to the
drug is increasing. Therefore, vaccine development became
one of the hopes for controlling this disease.
In this study a eDNA done of S mansoni encoding an
antigenic protein have been isolated from an adult worm
eDNA expression library. The library >vas screened with
chronic human serum. The clone was identified as a 786bp
(Ad3), and an ORF of 600 bp. PCR amplification, using two
primers flanking the ORF, of the genomic DNA of the adult
worm, suggested the presence of short intron sequences. DNA
homology analysis of Ad3 revealed the presence of very high
homology (98.7%) with a putative eggshell protein gene of S.
mansoni (SMEGGPRO). The presence of some nucleotide
insertions, deletion and substitutions resulted in a different
deduced protein designated as Sm22. The protein homology to
SI\1EGGPRO was 83.4%. In addition, it gave significant
homology to two vitelline precursors and putative eggshell
precursor of Fasciola hepatica. Sm22 may be an iso form of
SMEGGPRO isolated fi·om EgyptianS. mansoni strain.