الفهرس 
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Abstract
Liver fibrosis is a major handicapping disease. Hepatic stellate cells
(HSCs) are the pivotal cells in liver fibrosis pathogenesis. Mesenchymal
stem cells (MSCs) and hepatocyte growth factor (HGF) are participating in
liver fibrosis treatment; chitosan nanoparticles (CNP) provided the effective
delivery of HGF to its niche.
This work was designed to compare the effect of MSCs and HGF,
when given separately and when mixed together, in improving liver fibrosis;
using a rat model of thioacetamide (TAA)-induced liver injury.
Sixty male albino rats were used in this work and they were divided
into control group and the TAA induced fibrosis group that was further
subdivided into untreated fibrosis group, MSCs treated group, HGF
incorporated CNP (HGF-CNP) treated group, MSCs + HGF-CNP treated
group and CNP treated group. The livers were removed from sacrificed rats
and processed for staining with H&E, Masson`s trichrome and
immunohistochemical staining for alpha smooth muscle actin (α–SMA) and
proliferating cell nuclear antigen (PCNA). Fluorescent microscope was used
to detect PKH26 labeled MSCs.
The present study revealed that MSCs when mixed with HGF-CNP had
potent effect in improving TAA induced liver fibrosis as compared with the
moderate effect of MSCs or HGF-CNP alone. In the other hand CNP
achieved slight improvement in TAA induced liver fibrosis.
Key words: Liver fibrosis, MSCs, HGF, CNP, HSCs, TAA