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Abstract This study was carried out at a private apiary in Meat-Fares Village, Bani Ebaied Region, El-Dakhlia Governorate, Egypt, 25 km. from Mansoura city in the period from first of February, 2011 to the end of September, 2013 to investigate the following points: 1. Comparison of queen rearing methods between queen less colonies, a modified queen right colonies and a modified queenright colonies feed a natural queen pheromone inhibitor. 2. The Influence of number of brood combs in the mating nuclei on the oviposition period of the mated queen. 3. The influence of Oriental hornet (Vespa orientalis) attacks on mating of virgin queens. 4. The influence of queen age on colony tendency to swarm, number of combs covered with bees and honey production. 5. The influence of feeding sugar syrup methods on Brood production in honey bee colonies. Results could be summarized as follows: 1. Comparison of queen rearing methods between queen less colonies, a modified queen right colonies and a modified queenright colonies fed a natural queen pheromone inhibitor: A special Langstrouth hive with 12 frame size was prepared. The hive was divided from theinside with a vertical queen excluder to two sections and every section had its separate entrance section (a): the queen with three young brood combs section (b): the orphan section (one open brood comb,four sealed brood combs, grafted queen cups frame, honey and pollen comb). Queen rearing was compared in the three methods. 1-1-Queen rearing in queenless colonies: a. The acceptance percentage of queen larvae: There was no significant difference in the average of accepted larvae within the group (p˃0.05) and the accepted larvaepercentage was (81%) while there was a significant difference in the average of accepted larvae within the number of patches (p˂0.05). Although that there was a negative relation between accepted queen larvae and number of batches. b. Average queen weight: There was no significant difference in the average of queen weight within the queenless group (p˃0.05) and the average of queen weight was (168.56 ± 4.09 mg) while there was a significant difference in the average of queen weight within the number of patch (p˂0.05). There was a positive relation between the average of queen weight and the number of patch. 1-2-Queen rearing in queenright colonies: a. The acceptance percentage of queen larvae: There was a significant difference in the average of accepted larvae within the group (p˂0.05) and the accepted larvaepercentage was (67%) also there was a significant difference in the average of accepted larvae within the number of patches (p˂0.05). While there was a positive relation between accepted queen larvae and number of batches. b. Average queen weight: There was no significant difference in the average of queen weight within the queenless group (p˃0.05) and the average of queen weight was (180.08 ± 3.41 mg) while there was a significant difference in the average of queen weight within the number of patch (p˂0.05). There was a positive relation between the average of queen weight and the number of patch. 1-3-Queen rearing in queenright colonies with natural queen pheromone inhibitor (lemon juice): a. The acceptance percentage of queen larvae: There was no significant difference in the average of accepted larvae within the group (p˃0.05) and the accepted larvaepercentage was (72%) while there was a significant difference in the average of accepted larvae within the number of patches (p˂0.05). Also there was a positive relation between accepted queen larvae and number of batches. b. Average queen weight: There was no significant difference in the average of queen weight within the queenless group (p˃0.05) and the average of queen weight was (182.16 ± 3.04 mg) while there was a significant difference in the average of queen weight within the number of patch (p˂0.05). Also there was a high positive relation between the average of queen weight and the number of patch. |