الفهرس 
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Abstract
In this study, the immunomodulatory activity of thymoquinone and its interaction with cytokines gene expression was investigated in 16 New Zealand rabbits as a model for lab animals. The rabbits were divided into 4 groups each of 4 rabbits. The first group was injected with thymoquinone; the second was injected with S. aureus, the third was injected with thymoquinone solvent (control) and the fourth received saline (another control). Blood samples were collected at 3, 12, 24 and 48 hr. and 7days for detection the gene expression of IL-1β, TNF-α, IFN-γ and IL-10 and MHC-II. Blood samples were also collected from the first and fourth group at the 7th, 14th and 21st day for assessment of phagocytic activity, bactericidal activity and nitric oxide production. After 21 day, rabbits in the first and fourth groups were challenged with MDR S. aureus then, blood samples were collected at 3, 12, 24 and 48 hr and 7days for detection the gene expression of IL-1β, TNF-α, IFN-γ and IL-10 and MHC-II. The obtained results showed that:
I. Thymoquinone has no effect on peripheral blood mononuclear cells (PMNCs) morphology in either group. There was no change in cell morphology of isolated PMNCs in the thymoquinone treated group and control untreated group. II. Effect of thymoquinone on functional changes of PMNCs 1. Phagocytic activity The phagocytic capacity was determined in isolated peripheral blood mononuclear cells of both thymoquinone treated group and control. Cells contained ≥ 15 C. albicans were calculated and the percentage of these cells was determined. The phagocytic capacity was higher in cells isolated.