الفهرس 
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Abstract
In recent years, multidrug resistant virulent strains of E. coli
are circulating in broiler flocks and are implicated in cases of high
mortalities causing significant economic losses in poultry industry.
Therefore, the goal of the current study was the detection of the
most important virulence genes among MDR E. coli isolates from
different sources with relation to their resistance genes.
In the present work, 242 specimens were collected from
different sources (150 broiler organs with a previous history of
colibacillosis, 61 chicken by-products and 31 water tank samples)
during all seasons of the year from March 2011 to July 20 l3 in
Sharkia province,[Summer (84), autumn (54), winter (38) and
spring (66)].
Microbiological analysis of the collected samples revealed that
E. coli was recovered with a percentage of 20.2%; 22.7% from
broiler organs, 14.8% from broiler by-products and 19.4% from
water tanks.
Sas so breeds revealed the higher recovery rate of E. coli
isolates (25.7%). Moreover, the highest isolation rate of E. coli was
in summer (33.3%).
APT 20 E protocol was applied on randomly selected E. coli
isolates from different sources as a recent standardized and efficient
identification protocol. Results of APT 20 E confirmed those of the
conventional biochemical identification of E. coli isolates.
Serotyping of E. coli isolates revealed 8 different serotypes from
different sources with a dominance 026:K60 and 0114:K90