الفهرس 
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Abstract
The antiviral and cytotoxic activity of etanolic extract of Salvadora persica(L.) roots was investigated on bovine herpes-1 (BHV-1) infection in vitro. An ethanolic extract of Salvadora persica was used in two concentrations. MDBK cells grown on Minimum Essential Medium (MEM) were used for virus propagation and titration. The effects of two concentrations of Salvadora persica on viral growth in MDBK cells as well as cytolytic activity of BHV-1 were evaluated at different times pretreatment ,simultaneouslly and after adsorption. The results demonstrated that the safest concentration (non cytotoxic) of the plant extract under study on MDBK cells was 0.1 mg/ml.The plan extract showed a significant antiviral effect against BHV-1 at simultaneous treatment experiment. The virus titeration assay showed a marked decrease from 105 TCID50/ml to 103 TCID50/ml in adsorption step simultaneouslly incubated with Salvadora persica extract . In contrast, the plan extract didn’t show any activity against BHV-1 neither in pre- nor post- treatment experiments. Indeed, the present study confirms that ethanolic extract of Salvadora persica roots had an antiviral effect against BHV-1.