الفهرس | Only 14 pages are availabe for public view |
Abstract The present work was aimed to examine the effect of Wheat grass (Triticum aestivum), Maize grass ( Zea mays ), Barley grass ( Hordeum vulgare) and their blend on amelioration the hepatotoxicity in CCl4 injected rats and raising of immunity. For this purpose, the studies included about thirty mature albino rats weighing (220±10) g.B.Wt. each, were used and divided to 6 equal group, one was kept as control -ve group. The other 5 groups were injected s/c by carbon tetrachloride (CCl4) in olive oil 50% (2ml / kg.B.Wt.). Administration of single dose per day of CCl4 to a rats, twice a week for 2 weeks to induce hepatotoxicity in laboratory animals was carried out. Group (2): Control positive (+ve), in which rats were inflicted with hepatotoxicity by injection with CCl4, were fed on basal diet for 28 days. Group (3): CCl4 hepatotoxicity rats were fed on basal diet containing 5 % barely grass for 28 days. Group (4): CCl4 hepatotoxicity rats were fed on basal diet containing 5 % wheat grass for 28 days. Group (5): CCl4 hepatotoxicity rats were fed on basal diet containing 5 % maize grass for 28 days . Group (6): CCl4 hepatotoxicity rats were fed on basal diet containing 5 % of combination of all plants for 28 days (1:1:1). At the end of experimental period (28 day), animal were scarified. Blood samples were collected to determine the following parameters: Antioxidant Enzymes (SOD,CAT,GST), level of serum liver enzymes (ALT,AST,ALP) and AST/ALT, serum total protein, serum albumin, serum globulin ,total bilirubin , total cholesterol ,triglycerides, lipoprotein fractions (HDL-c, LDL-c and VLDL-c), atherogenic index(AI) ,Red blood cell (RBC), Hemoglobin (Hg), Hematocrit (Hct), Mean cell volume (MCV) , Mean corpascular hemoglobin (MCH), Mean corpascular hemoglobin concentration (MCHC) , Red blood cell distribution width (RDW) , Platelets , Mean platelet volume (MPV) ,Lymphocytes (LYM) , Granulocytes (GRAN) and Monocell immune distribution (MID).Liver,kidney,heart,lungs and spleen were kept in (10%) formalin solution for histopathological examination. |