الفهرس 
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Abstract
In this study, prevalence of Cryptosporidium species among animals at
Sharkia Province was investigated. Out of 150 examined animals (50 cattle,
50 sheep and 50 goats), 41 (27.33%) were positive by faecal examination. The
incidence of cryptosporidioisis was 40%, 24% and 18% in examined cattle,
sheep and goats, respectively. Calves were more infected than other animals.
For characterization of the structure and transmission of natural and
laboratory-propagated isolates, a collection of archived human and animal
isolates of C. hominis, C. parvum and C. meleagridis were analyzed by deepsequencing
polymerase chain reaction products amplified from a polymorphic
sequence on chromosome 1. Thousands of screened 200-nucleotide sequences
were analyzed to compare the diversity among samples, to assess the impact
of laboratory propagation on population complexity and to identify
taxonomically mixed isolates. Contrary to our expectation, repeated
propagation in animals did not reduce intra-isolate diversity nor was diversity
associated with host species. Significantly, in most samples, sequences
characteristic of a different species were identified. The presence of C.
hominis alleles in C. parvum and C. meleagridis isolates confirms earlier
reports of mixed isolates and raises the possibility that the host range of C.
hominis is broader than typically assumed. In a genetically divergent isolate
of C. parvum, a majority of sequences was found to be recombinant,
suggesting that this genotype originated from a C. parvum × C. hominis
recombination event.
GP60 sequences analysis of Thailand samples of six HIV-human cases
and four calves faecal samples infected with cryptosporidiosis, showed that