الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
smaller than 100 nm (nano =n=10-9) in at least two dimensions. At a nanometer scale, nanoparticles acquire a high surface area to volume ratio and novel physical, chemical and biological properties that are different from those of individual atoms, molecules and bulk materials. Rapid advances have been achieved in applications of nanotechnology in medical uses, with the emergence of a field termed “nanomedicine” which has potential impact on the prevention, early and reliable diagnosis and treatment of diseases. The main application areas of nanomedicine are; cancer diagnosis and therapy and delivery of drugs. Neuroblastomas, which account for 97 percent of all neuroblastic tumors, are the most common extracranial solid malignant tumor diagnosed during the first two years of life, and the most common cancer among infants younger than 12 months. They are most remarkable for their broad spectrum of clinical behavior, which can range from spontaneous regression, to maturation to a benign ganglioneuroma, or aggressive disease with metastatic dissemination leading to death. In this study, we used 2 types of neuroblastoma cell lines the SK-N-AS and the SK-N-DZ, which are known for the poor treatment prognosis with the known treatment regimens. Also the choice of the iron core nanoparticles is due to the known magnetic criteria of iron nanoparticles which is expected to have an adjuvant effect with radiotherapy to maximize the radio effect on cells containing the iron nanoparticles. SK-N-AS & SK-N-DZ cell lines were maintained in modified DMEM media supplemented with 10% heat-inactivated fetal bovine serum and 1% Penicillin-Streptomycin Solution. The Cells were incubated in a humidified 5% CO2 atmosphere at 37°C in a SANYO Safecell UV incubator. MTS viability test was first performed on SK-N-AS & SK-N-DZ cells in the absence of iron core nanoparticles in a 96 well plate at concentrations 3000, 4500, 6000, 7500, 9000 & 12000 cells/well. Absorbance was read with spectrophotometer to determine the cell concentration to be used. Then MTS assay was done again using SK-N-AS & SK-N-DZ cells at a concentration of 9000 cells/well with added iron nanoparticles at concentrations 0.01, 0.1, 1, 5, 10 μM/ml to determine the iron nanoparticles concentration to be used. Absorbance of the wells was read using spectrophotometer at 490 nm.