الفهرس 
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Abstract
This work planned to study clinical, haematological and biochemical studien as well as isolation and identification of the causative agent in some respiratory affections in cattle .
The diseased animals were grouped according to the cause of respiratory affection after laboratory investigation of oropharyngeal swabs into two groups:
Group I : included 75 feedlot cattle of about 2 years – old at Kalubia province , from the 55 showed fever, nasal discharge, coughing, conjunctivitis , inappetence , swollen joints with a history of no response to symptomatic treatment. Laboratory samples were collected before and after tylosine injection for 3 successive days.
Ultrasonogram of affected lungs showed inflammed and thickened perital and vascered pleura. Laboratory investigation of nasal swabs revealed 6 isolates of mycoplasma, 5 isolates from these were identified as Mycoplasma bovis and confirmed by using PCR technique.
Blood haematology findings recorded significantly dropped Hb, RBCs and PCV values and elevated total leucocytic, neutrophil and monocyte counts in infected animals, compared with control.
Biochemical analysis of serum samples from infected animals showed significantly decreased of total protein, albumin, α1, β2, γ1 and γ 2-globulines, total immunoglobulines and A/G ratio. On the other hand, malondialdihyde, α2- globulin and β2 – globulin were significantly increased.
Group II: contained 213 fattening calves of about 2-years- old in two private farms at Tall-Hewin and Bilpase – Sharkia province; from these animals 109 calves were suffering from respiratory manifestations in the form of cough, rapid respiration, dyspnea, nasal discharge and fever. Animals were examined clinically and laboratory, samples were collected and used for isolation and characterization of the causative agent and haematological and biochemical analysis.
IBR virus was isolated from nasal swabs on tissue culture (MDBK), identified and confirmed by virus neutralization test and PCR technique.
Blood haematological findings revealed significant increase in RBCs count and PCV% as well as liver enzymes (GPT, GOT and LDH) in IBR infected calves compared to control. There were also significant leucocytosis and neutrophilia in IBR infected calves compared to control animals.
Analysis of serum protein and electrophoresis showed significant decrease in total protein, albumin, α1, total α, total γ protein and A/G ratio.