الفهرس | Only 14 pages are availabe for public view |
Abstract The present study aiming to determine the biodiversity of Rhizobium leguminosarum symbiovar Viciae at some Egyptian governorates which its soil varied at fertility, salinity and pesticides applications. To achieve this goal; thirteen Rhizobia isolates were collected from ten governorates and subjected to the following biological assays: Growth rate and morphological character levels, i.e. salinity tolerance, herbicide resistance, antibiotics resistance and carbon source utilization. Biochemical differentiation levels such as indol acetic acid production, catalase enzyme and protein banding pattern. Molecular level such as plasmid profile, Nod c gene detection and RAPD – PCR for its DNA content. The principle objectives of this study are isolation salt and herbicide tolerant Rhizobium which can help their host plant to support adverse ecological conditions and investigate the effects of antibiotics, herbicide and salt on growth of Rhizobium isolates. Furthermore detect the diversity of these populations by using several biochemical and molecular approaches to observe their diversity and their relationship with different isolates. |