الفهرس 
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Abstract
In this study, a total of 250 samples were collected from living (50) and slaughtered broilers (200) during the period from October 2012 to November 2013 and examined by different protocols for detection of Campylobacter spp. The results revealed that 37 (14.8%) out of 250 samples were positive for Campylobacter spp. the rate of detection of Campylobacter spp. from drip (n=19), fecal (n=50), bile (n=50) and cecum (n=80) samples was 31.6%, 28%, 14% and 12.5% respectively, while Campylobacter spp. couldn’t be detected in examined neck skin (n=21) and liver (n=30) samples. Out of total 200 samples collected from slaughtered broilers, Campylobacter spp. were isolated from 23 (11.5%) of examined samples. Seasonal distribution of Campylobacter spp. was (21.2%) in summer followed by (17.1%) autumn and (13.6%) in spring. While Campylobacter spp. couldn’t be detected during winter season in this study. The PCR method directly from enrichment broth yielded the highest detection rate of Campylobacter spp. in 50 fecal samples (28%) followed by passive filtration method (16%) while the selective enrichment method failed to isolate Campylobacter spp. in any of examined samples. Results revealed that all 23(11.5%) positive samples with conventional isolation method were positive by PCR method using 23s rRNA gene of Campylobacter spp. Genotyping of C. coli and C. jejuni spp. by detection of hip O gene for Campylobacter jejuni and glyA gene for C.coli demonstrated that the distribution of C. jejuni and C. coli in the examined broiler samples in relation to the total no. of isolates was 4 (10.8%) and 19 (51.4%), respectively. While 14 (37.8%) were non-jejuni non-coli spp.