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العنوان
Extraction of active antimicrobial compounds from marine macro algae collected from some egyptian mediterranean seashores /
المؤلف
Hassan, Abdel-Aal Abdel-Khaliq Abdel-Aal.
هيئة الاعداد
باحث / عبد العال عبد الخالق عبد العال حسن
.
مشرف / علا حموده ابراهيم
.
مشرف / حسام مختار عبد العظيم
.
الموضوع
Marine algae egyptian mediterranean seashores.
تاريخ النشر
2014.
عدد الصفحات
118 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
1/1/2014
مكان الإجازة
جامعة بني سويف - كلية العلوم - النبات والميكروبيولوجي
الفهرس
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Abstract

Members of the class Ulvophyceae such as Ulva fasciata Delile, Ulva intestinalis Linnaeus, Ulva lactuca Linnaeus, Ulva pertusa & Ulva rigida and classs Phaeophyta like Laminaria sp. were collected from tidal and intertidal zone of Mediterranean sea shores during Winter and Summer 2011 and extracted in ethanol(70%). The total summation of the recorded total protein increase in the order: Ulva pertusa < Ulva fasciata < Ulva intestinalis < Ulva rigida < Laminaria sp. < Ulva lactuca, with percentage; 29.1, 28.7, 27, 24, 21.9 and 17.6%, respectively.The total summation of the recorded total carbohydrate increase in the order: Ulva lactuca < Laminaria sp. < Ulva rigida < Ulva intestinalis < Ulva fasciata < Ulva pertusa, with percentage; 55.6, 52.69, 49.63, 47.93, 44.2 and 43.79%, respectively.The total summation of the recorded total ash increase in the order: Ulva lactuca < Ulva fasciata< Ulva rigida < Laminaria sp. < Ulva intestinalis < Ulva pertusa, with percentage;17.6, 17, 15.3, 14.8, 14.6 and 10.3%,respectively.The total summation of the recorded total moisture increase in the order: Ulva pertusa< Ulva rigida <Ulva intestinalis < Ras elbar1 < Ulva fasciata < Ulva lactuca, with percentage;16.27, 10.40, 9.93, 9.63, 9.28 and 8.50% respectively. The total summation of the recorded total crude fat increase in the order Laminaria sp. < Ulva lactuca < Ulva rigida < Ulva fasciata < Ulva intestinalis & Ulva pertusa >, with percentage; 0.98, 0.7, 0.67, 0.60, 0.54 and 0.54%respectively. Phytochemical screening showed the presence of carbohydrates and/or glycosides, sterols and/or triterpenes and traces of tannins in all marine algae under investigation, the presence of both free flavonoids and/or combined flavonoids in all marine algae under investigation, Saponins are absent in all Ulva sp. under investigation, Cardiac glycosides, anthraquinones and alkaloids are absent in all the organs under investigation and volatile substances are absent all Ulva sp.. Antimicrobial activity of Ulva sp. was tested against (10 Gram +ve bacteria,10 Gram –ve bacteria and 10 unicellular Filamentous fungi).The antimicrobial activities were expressed as zone of inhibition and minimum inhibitory concentration (MIC).Identification of Compounds from crude extract of Ulva sp. carried by LC/MS technique. Finally Ulva sp. could serves as useful source of new antimicrobial agents.
Keywords: Marine algae, Ulva sp. (Ulva species), Minimum inhibitory concentration (MIC) and LC/MS (Liquid chromatography/Mass spectroscopy).