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Abstract Two experiments (one of them field experiment and the other pot experiment ) were carried out at the Experimental Farm of the Faculty of Environmental Agriculture Science, El- Arish, Suez Canal University, during the winter seasons of 2009/2010 and 2010/2011. The first experiment aimed to study the effect of three nitrogen sources (ammonium nitrate 33.5% N, urea 46 N % and ammonium sulfate 20.6 %N beside control ) and two nitrification inhibitors (thiourea and DCD) on kohlrabi crop. At 60 days from planting, plant sample were randomly taken to determine plant growth parameters and yield as well as at harvest, sample of stems were taken to determine the content of nitrate and nitrite. The second experiment was done on lettuce plant and aimed to study the effect of three nitrogen sources (ammonium nitrate, urea and ammonium sulfate) and two nitrification inhibitors (thiourea and DCD), vegetative growth, yield and heads content of nitrate and nitrite. The experimental design was split-plot in a randomized complete blokes (RCBD) With three replication for the two experiments. FIRST EXPERIMENT: EFFECT OF NITROGEN FERTILIZER SOURCES AND NITRIFICATION INHIBITORS ON GROWTH, YIELD AND NITRATE CONTENT OF KOHLRABI PLANT (Brassica oleracea var gongylodes). The obtained results could be summarized as follows: 1. Vegetative growth characters and average stem weight SUMMARY and CONCLUSION 1.1. Effect of nitrogen fertilizer sources: Application of urea gave the highest values of vegetative growth characters such as plant highest, root length, root fresh weight and leaves number/plant compared to control treatment in the both growing season. Also urea treatment gave the highest values of stem length, stem diameter, stem weight and average stem weight comparing to control treatment in the both seasons. 1.2. Effect of nitrification inhibitors Using DCD nitrification inhibitors gave the highest values of vegetative growth characters such as plant highest, root length, root fresh weight and number of leaves/plant comparing to control treatment in the both seasons. Also DCD treatment gave the highest mean values of stem length, stem diameter, and stem weight and average stem weight comparing to control treatment in the both seasons. 1.3. Effect of interaction: The interaction between urea and DCD gave the highest values of vegetative growth characters such as plant highest, root length, root fresh weight and number of leaves /plant in comparison with control treatment and the other interaction treatments in the both seasons. In addition urea combined with DCD gave the highest values of stem length, stem diameter, stem weight and average stem weight comparing to control and other interaction treatments in the both seasons. SUMMARY and CONCLUSION 2. Dry weight: 2.1. Effect of nitrogen fertilizer sources: Fertilizing kohlrabi plant with urea gave the highest values of dry weight such as root dry weight, leaves dry weight and stem dry weight compared to control treatment. 2.2. Effect of nitrification inhibitors: Application of DCD gave the highest mean values of dry weight such as root dry weight, leaves dry weight and stem dry weight comparing to untreated control. 2.3. Effect of interaction: Addition of urea combined with DCD was the best interaction gave the highest values of dry weight such as root dry weight, leaves dry weight and stem dry weight compared to control treatment in the two studied seasons. 3. Photosynthesis pigments 3.1. Effect of nitrogen fertilizer sources: Urea treatment gave the highest chlorophyll a content comparing to control treatment in the first season, but ammonium nitrate treatment gave the highest content comparing to control treatment in the second one. Regarding chlorophyll b, ammonium sulfate treatment gave the highest values comparing to control treatment, while urea treatment gave the highest content of total chlorophyll (a+b) comparing to control treatment in the both seasons. In addition, carotenoids ammonium sulfate treatment SUMMARY and CONCLUSION gave the highest carotenoids content in kohlrabi leaves comparing to control treatment in the both seasons. 3.2.Effect of nitrification inhibitors: Application of DCD as nitrification inhibitors gave the highest content of chlorophyll a, chlorophyll B, chlorophyll a+b and carotenoids compared to untreated control in the two tested seasons. 3.3.Effect of interaction: The interaction between ammonium nitrate and DCD gave the highest values of chlorophyll a, b and carotenoids content comparing to control treatment in the both seasons of study. On the other hand the interaction between urea and DCD gave the highest content of total chlorophyll a+b compared to control treatment in the both seasons. 4. Yield quality 4.1. Effect of nitrogen sources: Fertilizing kohlrabi plant with ammonuim nitrate gave the highest values of T.S.S of leaves in first season compared to the other nitrogen sources, while control treatment gave the highest content in the second one. In addition, application of urea gave the highest NO3 and NO2 contents in both seasons comparing to control treatment which gave the lowest values in this respect. On the other hand carbohydrates contents was not significantly SUMMARY and CONCLUSION affected by all studied nitrogen sources in the both seasons of study. 4.2. Effect of nitrification inhibitors: T.S.S and carbohydrate contents in kohlarbi leaves were not significantly affected by all tested nitrification inhibitors in the both seasons, while control treatment gave the highest content of NO3 and NO2 compared to DCD as NIs treatment in the both seasons. 4.3. Effect of interaction: The interaction between ammonuim nitrate without nitrification inhibitors gave the highest values of T.SS in first season, while all interactions treatments between control, nitrogen fertilizer and nitrification inhibitors gave the highest values in second season. While, the interaction between urea treatments without nitrification inhibitors gave the heights values of NO3 and NO2 and control treatment recorded the lowest values in this respect in both seasons of study. In addition, carbohydrate contents did not show any significant differences, except the interaction between urea and thiourea and the interaction between ammonuim nitrate with DCD or without nitrification inhibitors in the second season. SUMMARY and CONCLUSION SECOND EXPERIMENT: EFFECT OF NITROGEN FERTILIZER SOURCES AND NITRIFICATION INHIBITORS ON GROWTH, YIELD AND NITRATE CONTENT OF LETTUCE PLANT (Lactuca sativa var. capitata ) The obtained results could be summarized as follows: 1. Vegetative character and average head weight 1.1. Effect of nitrogen fertilizer sources: Urea fertilizers treatment gave the highest values of vegetative growth characters such as plant height, root length, fresh weight of root and leaves and number of leaves/plant comparing to unfertilized control treatment in the both seasons, also adding urea to lettuce plant gave the highest head height, head diameter, average head weight, and yield per fed. on comparing control treatment in the both seasons. 2.1. Effect of nitrification inhibitors: Application of thiourea as nitrification inhibitors to lettuce plant gave the highest values of vegetative growth character such as plant height, root length, fresh weight root and leaves and number of leaves/plant comparing to control treatment in the both seasons. In addition, DCD treatment gave the highest head height, head diameter, average head weight, and total yield comparing to control treatment in the both seasons. SUMMARY and CONCLUSION 3.1.Effect of interaction: The interaction between urea and thiourea gave the highest values of vegetative growth character such as plant height, root length, fresh weight root and leaves and number of leaves/plant comparing to control treatment in the both seasons. Also urea combined with DCD was the best interaction treatment which gave the highest values of head height, head diameter, average head weight, and total yield per fed. comparing to control treatment (control N X control NIs ) in the both seasons. 2. Dry weight 2.1. Effect of nitrogen fertilizer sources: Fertilizing lettuce plant with urea gave the highest values of dry weight such as root dry weight, leaves dry weight and stem dry weight in comparison with un fertilized control in the both seasons. 2.2. Effect of nitrification inhibitors: Nitrification inhibitors as DCD treatment gave the highest values of dry weight of different plant parts, i.e., root dry weight, leaves dry weight and stem dry weight of lettuce plant compared to control treatment in the both seasons. 2.3.Effect of interaction: Urea combined with DCD treatment gave the highest values of lettuce plant dry weight as root, stem and leaves/plant comparing to control treatment in the both seasons of study. SUMMARY and CONCLUSION 3. Photosynthesis pigments 3.1.Effect of nitrogen fertilizer sources: Using urea as nitrogen sources gave the highest content of chlorophyll a,b, total chlorophyll (a+b) and carotenoids comparing to control treatment in the both seasons, except chlorophyll a which did not reach the S% level of significance. 3.2.Effect of nitrification inhibitors: Application of thiourea to lettuce plant gave the highest content on chlorophyll a and chlorophyll a+b in leaf tissues of lettuce plant comparing to control treatment in two seasons, but DCD treatment gave the highest chlorophyll b and carotenoids comparing to control treatment in the both seasons of study. 3.3. Effect of interaction: Fertilizing lettuce plant with urea combined with thiorurea was gave the most favorable interaction treatment for increasing the contents of chlorophyll a, chlorophyll b, chlorophyll a+b and carotenoids in lettuce leaves comparing to control treatment in the two seasons. 4. Yield quality 4. 1. Effect of nitrogen fertilizer sources: Nitrogen source was not significantly affected TSS% in lettuce leaves in the first season, while ammonium sulfat treatment gave the highest TSS values in the second one. On the other hand, SUMMARY and CONCLUSION urea treatment gave the highest NO3 and NO2 content in the both seasons comparing to control treatment which gave the lowest values in this respect. While, all nitrogen sources did not show any significant effect regarding carbohydrates content in lettuce heads. 4.2. Effect of nitrification inhibitors: No significant effect was dectected due to nitrification on TSS% and carbohydrate content of lettuce heads in the two tested seasons, while, NO3 and NO2 was at its minimum values when lettuce plant treated with thiourea compared to untreated plants with nitrification inhibitors. 4.3. Effect of interaction: The interaction between all nitrogen sources combined with nitrification inhibitors gave the heights values of TSS%. Also the interaction between urea without nitrification inhibitors gave the heights values of NO3 and NO2 while control treatment gave the lowest values of NO3 and NO2 in the lettuce heads in both seasons of study. No significant differences were found among all the interaction treatments regarding carbohydrate content, except the interaction between urea with DCD or thiourea which increased the content of total carbohydrates in the first and second seasons, respectively. 771 SUMMARY and CONCLUSION CONCLUSION Under similar condition of this experiment. It could be concluded that, kohlrabi and lettuce plants fertilized with urea combined and DCD or thiourea being the most effective and favorable interaction treatments on increasing vegetative growth traits, dry weight of differente plant parts. Also these treatments increased average head weight and total yield per feddan in lettuce in both seasons. Application of thiourea or DCD combined with control (nitrogen source) followed by ammonium sulfate or ammonium nitrate combined with thiourea or DCD were the recommended interaction treatments that did not lead to excessive nitrate and nitrite contents in edible portions of kohlrabi and lettuce plant |