الفهرس 
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Abstract
Pseudomonas aeruginosa is one of the most common gram-negative bacteria
found in nosocomial infections. It is found to be an important causative agent for
nosocomial pneumonia cases, hospital acquired urinary tract infections, surgical
wound infections and bloodstream infections.
Multi-drug resistant P. aeruginosa have increased rapidly and became a major
problem in the area of infectious diseases.
The present study was carried out during the period from December 2012 to
December 2013 and involved 49 P. aeruginosa strains isolated from 307
hospitalized patients with evidence of nosocomial infections to detect the role of
efflux pumps in P. aeruginosa isolated from Suez Canal university hospitals in
Ismailia, as one of the mechanisms of resistance in this organism.
Specimens included urine, sputum, blood and pus, collected from different
hospital wards. The collected specimens were processed in the Microbiology
Department, Faculty of Medicine and Suez Canal University for the isolation and
identification of P. aeruginosa.
Pseudomonas aeruginosa was identified according to the following: gramnegative rods that were non lactose fermenting on MacConkey’s agar, oxidase
positive and produced pyocyanin pigment on Pseudomonas P agar.
Pseudomonas aeruginosa were isolated from 49 clinical samples representing
15.9% of all nosocomial infections in