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Abstract !’1e present work aims to study t:.1e rhiz~>spherio .t.e.r:¥.-:s) ”:Jaladi” and ”Aeskawi” VRrleties. .L’he e1Ll0id2.tion of t.\e ef’feot of solu.ble, VOl.:! tile and e0.seous of elth.e.r germi::1a·~ing S6eds or roots of t.’:le experimental plc.nta on the f;_uc;al population in the root z:>:<e, as well as t!:J.e effect of selected rhizospherio f~>gi on the internal oontent of the experlment,J.l plal’lts i1ave been also stLtdied. 1- l’late dilution method was Llaed for the isolatio::J. of r:<izospheric myooflora of t’1e experimental plants. ReS\.llts have sh.:xom t.hu.t the total nttm0ar of .fLwgi .L”l the l.’oot zone was more t;h.”’n elsw!lere in t:1e soil. 2- I::1 General, BJJ....~9.B.Ltf! .s_t_q.J,Q.<”’.J/.G.r. represeated the nost a~und.J.nt fungus i..’l both r:1izosphere or soil whetl1er ef:Z::Jot, i.e. °Kafr El-Da’11::lr” O’cra variety Vi’as o:l:-.l•acterized by Al,;~e_:;:.zw.r.Jil a~t..E!..r.n.:JS_a.. while ”Gold Coast 11 Olc:r:-a variety was charac t cr izad by f.,l1)..2:.0J?...Ll,..S. st.o.l.c.a . .if.e~.t: as ·o11e most abL.mdan.t fllJ16us • whet:1er r;J.ised on clay or 1oa... soil. On the other hand, ”BalaCli’.’ Lupins variety crolL in loam soil was characterized by the presence of Aspergillus .·:.>..o l11.·aceus as i;.1e •nest ab•.tvh:<:; fcm.gu::; L~ its :r.’lizo- ~_ .. _._ ... ~ plc:-.:’lt c;rowa in clay soil. 3- Cultural studies have bee~ carried out to evaluate the gravvth responses of the experLlen’t:al rhizosphsric fungi; i.e. A,ltern¥2..?. s),te:t;l:l:c”-tq_, /l..fl.J.J.Cr_g~:J.li.Y!. g_o_hrao.e.u,.s.1 RM.C\_~ sol~, Penio,iJJJ,.l..\c’i .~o),pJ!.ill.’ll and BJli.~.OJ>. .L !§. !’lJ_oJ.o. l. 1Jfer to the variation. of either enV’iro:-::::o:htO.l or physiological fP.otors. :~he following fundings were l’ecorded. b} ;;: • .ClLO .. lOI!~ prefered JUc’~ard’ s medium. Asparagin was prefered ’;)y A........ . -oo_h__r_o_._o_e__u_s .. . and sodiu:,l nHrate by .;[ • .a.o).ac’\i. and pGptone by r. s..Y.o),.o..PJ..~. e) L!altose proved to be t:1c most preferable carbon source to A· 1.\lli_ma.t_g., ~ •. o_oJlt’_a,.oeu.s. and J:.. cyclopiu.:n, sucrose by &· so_le~ni, whereas ::;taroh was the most preferable carbon source d) 2he ;rpt.Lilllm p;; V’altle fo~· ,t .• ~..i.o.l_of’lif§_I’. was 5. J, whereas that or the r<:’L1.’:1.il1L’l::; fLmgi was s. o. 4- c:ll’Ol11.’J.tographio a”12lysis of seed roo”G exudntes of 11Gold Coast” Okr2. Ve>.rist:• 1;:1.s shown t\at they oo.1tcdned -~:lc following ::J.dino acids ’J.lld actg;o.rs: glutctmio, leucine, n.rgn.aL1e, valine, oyctine, p:1.enylala:c1ine, se1<i’lr, ~1··~;-. isoleucine, QC alan.i.ne a:1cl two unidentified a’Jlino acid; raffinose, sucrose, gll.loosa and one W1ifa;1ti.fisd s·.tgar. Secrl and root exudates of tr.1a other experimentn.l Okra V’L’.riet y namely ”Ka.!r El-Da’:lar” contained gl11t2.<;tio, leucL1e, arganine, valine, o~·at ine, phenylalanin:’l, sar.L1e, glucine, isoleucine a:’ld two W1ideutif ied ” 1i:nCJ aoids. Seed and root exllfla~es of the same V2.riet,y oaTt:J.ined ~e following s:.v~o.rs: raffinose, sucrose, gl:.tcose, eellu~iose, and one :.nidentifisd suc;~1·. S:::ed cmd root exlld2.tes o’f the ot.~1ar experimental pl..,nt, i.e. WJa~adi” Lupins variety C0:1·~ai:1ed the following acnino aoids and sugars; t :•rosiae, ;;16 thionine, ,~alanine J-sole~cine, leucine, glll:.O.ilic 1 seri:1e, glllcinc, two u:.’lidant if .i.e d <’llllino <J.cids, ~;Ltcosa, oellllbiose, raff L:wS’:. a:’ld sucrose. Amino acids a:1d sugars detected L1. eitlLer aced alld root extldates of t.’le seooud ” I~escl:awi’’ :Lup.ins v,-.ri.oty vrere: t,YJ:<osine, raethiOl’lL’1e, ~alanine, isoleu. oLle, cystine • serine, gluc.Lw, tr yp~ophane, Ve>.l.i .. 1e, .r:~ffinose, SLtOrose, glll,~.otOEHl c.:1d oellubiOSEI.5- _;;:eel ’-’1d r•:JO~ e;;:ud’-lt3s of ’1)3~ axperimen·~i1l .’lc:”1~s st.L;lll.lated spore ger;uinc.tiJ:1 ·’.:1cl mycelial grovrtl< of t :u;; rhi zosphe ric fungi. 6- Gaseou.s a11.d volatile e.Y.:lld::\’iies c112.na.ted from ger.nLlati’lg seeds and roots stLnuL~·~ed spore germination of ·the e:::perimei1tal fi.Ulgi. 7- ·J’re<J.ting the pl<mts with fu:1.c;al sllspension resull;ed in supprassiOl1 of so<11e grD’.vth oriteria of the experi;ne:1t:::.l Pl.:.nts. Suppression could. b::: correlated with deora:::.sa L1 phosphorLts conte:1t of ”Gold Coast” Okra Vc’lriety .ind :i)!losphorus and nitrogen oo:1te:ybs of the second n;cctr El-Dawar” Okra V£>oriatj’, i.e. SIJ.ppression L1. t.i1e vi::;oLU· of Lupine plants oould J8 attributed t:o d ’:’ ’J.c•q-, c:r. L1 their nitrogen contents. 8- Eao~1 one of the axperimt’!ntal fungi e:x:ertad. iU:1ibii;)ry ef:>:eots on tJ1e growth of t.’lc other ~Gsociatetl flnlgi l.-1. oul~ure. 9- i’he oo,;mareial spray fertilizer ”s;louehan” e~ccrted dist:.trbance of th.e pl2.nt nr-o;ta’:Jolism whioh was rai’1Got8d 0:1 ·~:1c fLU’l,Sco.l popllb tion of ·~ :1e root zo:lS, the i:1.ternal OOi’lStitLl’bion and the root eXlldntes of ·~he eXperi:nental plants. |