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Abstract Digital image analysis (DIA) allows quantitative assessment of fibrosis on liver biopsy. This method has not been fully evaluated in practice. This study investigated using digital images analysis by software ImageJ of liver sections stained by sirius red (SR). Reproducibility of this technique was tested in comparison with the a semi-quantitative score of liver fibrosis as well as other markers of fibrosis including physiological markers of liver functions such as alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT). Also, it was tested compared to serum marker collagen IV (Col IV) and hepatic hydroxyproline. There was a gradual increase in the serum AST, ALT, ALP and GGT activities as well as collagen IV and hydroxyproline content with the increasing dose of CCL4 and advancing of stage of fibrosis. Hepatic hydroxyproline, serum Col IV and ALT showed higher accuracy than other physiological markers with area under curve (AUC) of 0.935, 0.955 and 0.902 respectively. Also, DIA of SR by ImageJ software had shown the best accuracy with AUC of 1.0. In conclusion, the use of ImageJ with semiautomatic color segmentation is a reliable and practical way of measuring collagen proportionate area. On the other hand, from physiological point of view, hydroxyproline, Col IV and ALT showed the high accuracy, sensitivity and specificity so it can be used for liver fibrosis diagnosis. |