الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 82 |  |  |
| | | from | 82 | | |
Abstract
Salmonellosis is one of the most common Zoonotic infections. The most important source for Salmonella infection is carriers of organisms without outward signs.
A total of 411 samples were collected from liver, gall bladder and lung of 137 slaughtered bovine and ovine as 63 slaughtered bovine and 74 slaughtered ovine. These samples were collected from abattoir in El- Minia Governorate and also collect a total of 180 rectal swabs were taken from apparently healthy bovine (90) and apparently healthy ovine (90). These samples were collected from different localities in El- Minia Governorate.
All samples collected were subjected to bacteriological, serological and PCR examination for isolation and identification of Salmonella species.
In the present investigation found that the prevalence rate of Salmonella isolates was 23.8% and 6.6% from slaughtered and apparently healthy bovine respectively.
Meanwhile the incidence of isolated Salmonella was 14.8% and 4.4% from slaughtered and apparently healthy ovine respectively.
Identification the isolates revealed that the prevalence of Salmonella serotypes which isolated from the internal organs of slaughtered bovine was [liver (15.8%), gall bladder (4.7%), lung (3.1%) and feces (6.6%)]. Meanwhile the prevalence rate of Salmonella serotypes which isolated from the internal organs of slaughtered ovine was [liver (10.8%), gall bladder (1.3%), lung (2.7%) and feces (4.4%)].
Comparing the different sites of sampling it was revealed that the liver was the most predilection site for Salmonella.
Serotyping of 36 isolated Salmonella revealed 5 different Salmonella serotypes from bovine and ovine.
Salmonella Typhimurium was the most prevalent serovar in both bovine and ovine followed by Salmonella Enteritidis but in this present isolated Salmonella Virchow, Salmonella Montividio and Salmonella Rubislow has been found in a moderate and low incidence.
In this present work five isolates were examined by PCR to determine the virulence invA gene. All 5 isolates were positive for invA gene sequence as indicated by the size of the PCR product in agarose gels (approximately 521 bp)
Generally most of the different serotypes of Salmonella isolates in the present work were highly sensitive to many chemotherapeutic agents as ofloxacin, rifampicin, norfloxacin, moderate sensitivity to neomycin and tobramycin while low sensitive to streptomycin, and trimethoprim sulphamethoxazol.