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Abstract Summary and Conclusion - 89 - V. SUMMARY AND CONCLUSION In current work chitosan was produced from Aspergillus brasiliensis under controlled and optimum growth conditions with the following main aspects: A. Cultivation of A.brasiliensis on potato dextrose broth (PDB) and date syrup extract as an alternative source for the cultivation of fungus. B. Extraction of chitosan from fungal cell wall and studying the effect of main environmental parameters i.e, pH, temperature and incubation time. C. Chemical and physical investigation of chitosan including; molecular weight, degree of deacetylation, solubility and identification through IR spectroscopy. D. Studying the antimicrobial activity of the produced fungal chitosan through different methods and techniques i.e., MICs, disc diffusion and bactericidal effect. E. Application of fungal chitosan as a natural food preservative and comparing its action versus potassium sorbate which act as chemical preservative. |