الفهرس 
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Abstract
Chronic periodontitis is a complex inflammatory disease, which is a major
cause of tooth loss. The risk for chronic periodontitis is influenced by
environmental and genetic factors. The present study was conducted to
investigate the association between the Vitamin D gene receptor
polymorphism and chronic periodontitis in a sample of Egyptians.
80 subjects were recruited for the study from the patient pool at the
dental clinics of the faculty of dentistry, Alexandria University. All subjects
were non-smokers, Egyptians and in good general health within the age range
of 20-55 years of age. Subjects were divided into two main groups:
• chronic periodontitis patients group (n= 11 males and 29 females with age
range of 21-53 years and mean age of 39.15 ± 5.21years).
• Healthy controls group (n= 19 males and 21 females with age range of 20-
55 years and mean age of 26.95 ± 5.25years).
Both patients and controls were age and sex matched.
The diagnosis was made on the basis of standardized clinical and
radiographic criteria.
Genomic DNA was obtained from peripheral whole blood. Genotyping
of the Fok-1and Taq-1 polymorphism of Vitamin D receptor gene was carried
out using polymerase chain reaction- with confronting two- pair primers
(PCR) analysis. Genotype and allele frequency distributions among the
periodontitis patients and controls were compared using chi squared and
Fisher exact tests (p<0.05).
The results of this study showed no statistically significant association
between chronic periodontitis and Fok-1and Taq-1 polymorphism of Vitamin
D gene receptor. In both control and patient groups, there was a strong
predominance of the allele F. The frequency of allele F was slightly higher in
controls (100.0%) compared to chronic periodontitis patients (97.5%). The
frequency of allele T was higher in controls (98.8%), compared to chronic
periodontitis patients (93.8%). However, the differences in allele frequency
distribution between both groups were found to be not statistically significant.