الفهرس 
Introduction and aim of the workOnly 14 pages are availabe for public view
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Abstract
The present study aims to compare some methods applied in the study of epidemiology and molecular variability of clinical isolates of K. pneumoniae isolated from Mansoura hospitals, Dakahlia governorate, Egypt and to confirm by classical and molecular laboratory methods hospital associated outbreaks due to K. pneumoniae isolates. In this study, a total of 300 clinical isolates were collected from different patients distributed among Mansoura hospitals, Dakahlia governorate, Egypt. Ninety six isolates were identified as Klebsiella pneumoniae using standard biological methods. Seventy isolates were obtained from urine samples, 9 isolates from each endotracheal tube, nose and throat, 8 isolates from blood, 6 from sputum and one isolate from each of the following wound, diabetic foot and coronary catheter. Different typing techniques were applied including antibiotic susceptibility testing and different molecular typing techniques including: plasmid profile after single digestion with EcoR1, RAPD analysis using two individual primers; AP3 and OPA13. In the present study, the plasmid was isolated from the ninety six clinical isolates of K. pneumoniae, plasmids were found in seventy eight (81.25%) of the ninety six K. pneumoniae isolates. Single digestion of plasmid DNA with EcoR1; revealed that sixty seven clinical isolates were distributed into twenty four patterns (T1-T24). Hence, Random amplified polymorphic DNA (RAPD) analysis was applied as another molecular typing method using two individual primers, AP3 and OPA13. OPA13 showed eighteen distinct patterns (RA1-RA18) representing 80 isolates. However, PCR amplification could not be obtained with 16 K. pneumoniae isolates. Primer AP3 showed more discriminatory patterns (R1-R43) representing 93 isolates and can be used individually for better interpretation in typing a large number of isolates. Epidemiological typing of 96 K. pneumoniae clinical isolates performed using phenotypic and molecular methods. There is solid relationship could be traced between the clinical isolates from the same source. So some studied strains have been shown to be epidemiologically related.