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العنوان
DNA fingerprinting and morphological description to identification of some maize inbred lines and its crosses /
المؤلف
Al-Rawi, Ahmed Rajab Mohammed.
هيئة الاعداد
باحث / أحمد رجب محمد الراوي
مشرف / أحمد نادر السيد عطية
مشرف / محمود سليمان سلطان
مشرف / محسن عبدالعزيز بدوى
مناقش / ألفت حسن الباجورى
الموضوع
DNA. Maize.
تاريخ النشر
2015.
عدد الصفحات
174 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة المنصورة - كلية الزراعة - قسم المحاصيل
الفهرس
Only 14 pages are availabe for public view

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Abstract

This study aimed to : Two field experiments were conducted out during the two successive seasons of 2012 and 2013 at the experimental station farm, faculty of agriculture, mansoura university, dakahlia governorate. The international union for the protection of new varieties of plants (upov, 2009) technique was used to evaluate the genetic purity of maize genotypes and their f1 single crosses. These genotypes were : r 39, p 97, b 73, inb.1021, inb.1004 and oh. 43.results indicated for undulation of margin blade of the leaf in the middle of plant the presence of 6 highly significant differences were detected in average of plant height excluding tassel (cm) among studied inbred lines and their crosses. The studied inbred lines could be ranged from 93.33 cm to 160.66 cm, while single crosses appear to range from 150.00 cm to 227.66 cm.Results revealed that mean squares of crosses were highly significant for all studied traits, indicating wide range of genetic variability among the studied crosses and this is primary requirement for further computation. Bboth general and specific mean squares were found to be highly significant for all studied traits, indicating the importance of both additive and non-additive genetic variance in determining the performance of these traits. gca/sca variances ratios were found to be greater than unity for 2 traits i.e. Plant height and 100-kernel weight, indicating that the additive and additive×additive types of gene action were greater importance in the inheritance of these 2 traits. While, gca/sca variances ratios were found to be lower than unity for other remaining traits i.e.days to 50% tasseling emergency, days 50% to silking emergency, number of rows/ear, number of kernels/row, grain yield/ plant and shelling percentage, indicating the performance of non-additive genetic variance in the inheritance of these traits.the obtained results showed that with 0p- b16 primer there were 9 bands had a range of size between 100-1200 b.p. These common bands were band no. 5 which was only in 1 single cross,band no. 6 and , band no. 7 which were only detected in 3and 4 single crosses, respectively, while band no. 8 which was only in 6 genotypes (2 inbred lines + 4 single crosses), band no. 9 which was only detected in 9 genotypes (5 inbred lines + 4 single crosses), band no. 10 recorded only in 6 genotypes (3 inbred lines + 3 single crosses), whereas band no. 11which was in all genotypes, except for 3 genotypes (1 inbred line + 2 single crosses), as do the band no. 12 which was in all genotypes, except for 9 genotypes (4 inbred lines + 5 single crosses), and finally band no. 13 which was in all genotypes, except for 8 genotypes (4 inbred lines + 4 single crosses). Morphological and bio-chemical traits used to identification genotypes of maize plants. When identification between genotypes, morphological methods using firstly, followed by the modern bio-chemical methods, especially when differences among morphological characteristics unclear. The technique polymerase chain reaction integrated is considered as effective system to identification genotypes. Thus, this study recommends using the pcr technique, despite the high cost.