الفهرس 
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Abstract
Accumulation of a lot of chitinous substances contained in shell of shrimp, crabs and others creates serious problems in environmental pollution in Egypt and many other countries. The present work was conducted to evaluate the potentiality of some fungal species, isolated from sediment soil and shellfish wastes. The study was extended to investigate the effect of partially purified chitinases on the growth of some phytopathogenic fungi in vitro. Twenty fungal species were isolated from ten samples of soil sediment and shellfish wastes. Penicillium chrysogenum showed the highest active fungal strain in the production of chitinase enzymes in SSF. Plackett-Burman design and response surface were used to determine which variables significantly affect chitinase production by P. chrysogenum. 1.5 ml colloidal chitin, NaNO3: 0.5, KCl: 0.06, CaCl2: 0.127, NaCl 0.01, pH: 4.2, temperature: 32°C, moisture level: 5.6 ml inoculum size: 1.9 (ml /g dry substrate) and incubation period: 6 days were found to be the optimum conditions for high chitinase production by P. chrysogenum. In vitro, antifungal activity of chitinase produced by P. chrysogenum against some phytopathogenic fung. The results demonstrated that the isolated fungal chitinase was highly effective enzyme against all tested phytopathogens.