الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
The bacterial diseases is one of these out breaks which led to great economic losses. The current study aimed to isolation and identification of these pathogens. In this study 180 Oreochromis niloticus samples were collected from different farms in Kafrelsheikh Governorate for isolation of bacterial pathogens. The bacterial isolates were identified by traditional methods then confirmed by PCR technique using specific primers. PCR confirmed 86 isolates of A.hydrophila at the molecular size of (103 bp) and 26 isolates of Ps.aeruginosa at the molecular size of (958 bp), 11isolates of Ps.fluorescens at the molecular size of (850 bp), 8 isolates of V.alginolyticus at molecular size (337 bp), 5 isolates of V.anguillarum at molecular size (429 bp), 28 isolates of S.iniae at molecular size (300 bp) and 8 isolates of S.agalactiae at molecular size (220 bp). Conclusion: A.hydrophila, P,aeruginosa, P.fluorescence, V.alginolyticus, V.anguilarum, S.agalactia and S.iniae were involved in the outbreak affecting fish farms in Kafrelsheikh governorate and using of PCR in diagnosis of bacterial diseases in aquaculture is very reliable, accurate and rapid method due to their high sensitivity and specificity for the pathogen detection and may assist in the prediction and control of potentially pathogenic microorganisms.