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Abstract These research was carried out on the Pleurotus ostreatus mushroom to study it’s antitumor , anti cancer antitoxicity, and antioxidant activities. The obtained result showed that Pleurotus ostreatus mushroom would be considered as a valuable source of antioxidant, antitumor and anticancer compounds. The proteins, phenol, polysaccharide compounds and fat content of Pleurotus ostreatus, were determined its extracts were tested as antitumor , anticarcinogenic and antioxidant agents by on mice tumor and in vitro cytotoxic activity against Hepatocellular carcinoma cell line (Hep G-2 cell line). Treatment of tumor-bearing mice with the extracts for 11 days restored the natural killer activity against tumor cells in mice, and on the hepatic human cancer. We are found that the Vicia faba plants treated with 10 g/kg, 20 g/kg spent straw increase in plant height, number and area of leaves, the number of nodules and lateral roots, dry weight of both stem and leaves, increased at 50 g/kg. Moreover, the amount of monosaccharide, disaccharide, polysaccharide, total sugar contents were significantly increased at 50 g/kg of both stem and leaves at the highest concentration at vegetative , flowering and yield stages. Also, an increasing in the protein and phenol contents at high concentration plants were compared with the control plant. Protein. plants treated with three different concentrations (0.5g/L, 0.25g/l and 0.125g/l) respectively, of the extracts. showed that the Pleurotus ostreatus mushroom was not mutagenic. Under the circumstances of this study, the results indicate that the mushrooms Pleurotus ostreatus extracts did not show any genotoxic or mutagenic effects, but are protect plant and are |