الفهرس 
Introduction
Transfusion of Blood and Blood ProductsOnly 14 pages are availabe for public view
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Abstract
Nucleic acid amplification is an essential step in many important molecular diagnostic assays. Whole genome amplification increases the amount of patient samples when they are available in only limited quantities. Target-specific amplification selectively enriches the gene(s) and organism(s) of interest, allowing detection and analy¬sis.
The effectiveness of NAT to detect low levels of the virus in early stage of infection, by increasing the amount of specific target present to a level that is easily detectable, and able to detect the virus during the window period. The presence of specific nucleic acid indicates the presence of the virus itself and that the donation is likely to be infectious. Hence It can reduce the risk of transfusion transmission infections.
NAT identify seronegative donors undergoing seroconversion during the donation period. For example Viral RNA is normally detectable within a few weeks of infection and persists for 6–8 weeks prior to antibody seroconversion. The detection of viral RNA may further reduce the risk of viral transmission through the transfusion of infected blood donated during the window period of antigen and antibody assays: i.e. when the results of viral antigen-antibody assays are negative, but viral RNA is positive .