الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 16 |  |  |
| | | from | 16 | | |
Abstract
Introduction: The valuable proteomic techniques which is a comprehensive quantitative descriptive study of the total proteins expression and their changes in a system can offer a more focused analysis and place the protein in their biological context (Twigt, Steegers-Theunissen et al. 2012, Ambekar, Nirujogi et al. 2013). A comprehensive examination of follicular fluid through proteomic techniques which represent the link between genes-protein and physiological/pathological processes and is a very attractive field of research and will provide a novel perspective for understanding the follicular fluid constituents and their effect on pregnancy outcome (Santos, Lodeiro et al. 2010).
Aim of the study:
• To identify and cataloging the important follicular fluid proteins for women of 35 years of age or older.
• To determine the type of follicular fluid proteins that may be used as a predictor for occurrence of pregnancy in infertile women of 35 years of age or older managed by in-vitro fertilization through proteomic biometrics.
Patients and Methods: Twenty one women matched our inclusion criteria, and signed an informed consent that was approved by the Institutional Review Board of Meharry Medical College, Nashville TN. Follicular fluid of women who got pregnant “Successful Group” (11 women); are those with proven clinical pregnancy by both serum βhCG (more than 5 mIU/ml) 14 days after embryo transfer and ultra-sonography detection of fetal cardiac pulsations thereafter were matched to “Failure Group” (10 women) who failed to become pregnant after two successive negative readings of βhCG (less than 5 mIU/ml) after 14 days and 16 days of embryo transfer respectively.
Inclusion Criteria:
- The follicular fluid samples collected from follicles of women over 35 years of age after extraction of their ova, who underwent their first cycle of in-vitro fertilization and diagnosed with only tubal factor infertility.
Exclusion Criteria:
- Follicular fluid samples from women less than 35 years of age.
- Follicular fluid of women who underwent their second cycle of in-vitro fertilization.
- Follicular fluid of women diagnosed with endometriosis, diminished ovarian reserve, ovulatory dysfunction, uterine factors, male factors and unexplained factors.
- Follicular fluid of women required donor semen or donor eggs, gestational carries.
Samples were analyzed by multidimensional chromatography coupled in line to nano-spray ionization mass spectrometry on an LTQ XL ion trap mass spectrometer. We used the Biomarker Analysis Program from PDQuest software for identifying protein constituents in pregnant and non-pregnant groups.
Results: Total numbers of 1024 proteins in all the analyzed specimens were identified. The pattern was consistent throughout the experiment and within each of the analyzed specimen.
Conclusion: The finding of such list of proteins could be a starting list for identifying the proteins of importance and to evaluate the contained oocytes; the results of our study may fill a noticeable gap in the knowledge of follicular fluid proteome.
Recommendations
We recommend building on our findings and results to support future projects aiming to pick up the simplest to identify, cheap yet reliable protein predictor out of the previously cataloging protein so that to both simplify and transform the process of oocyte quality evaluation into an objective process. We also recommend further study with large number of patient to support our finding.