الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Enterococci, an endogenous flora of intestinal tract, oral cavity and urinary tract of humans and animals are known to be relatively avirulent in healthy individuals, but have become important opportunistic pathogens, especially in hospitalized patients. They belong to group D streptococci.
Recent years have witnessed increased interest in enterococci not only because of their ability to cause serious infections like endocarditis, bacteremia, intra- abdominal and urinary tract infection (UTI), but also because of their increasing resistance to many antimicrobial agents.
Here in , the aim of this work was to spot the light on isolation and identification of enterococci from different sources and detection of vancomycin resistant genes among them. Thus, it could be achieved through microbiological identification of enterocooci by cultivation on selective media ( Slantez and Bartley medium), conventional biochemical tests (bile aesculin agar medium, tolerance to NaCl 6.5%, growth at variable degrees of temperatures 10-45º C, growth at high PH 9.6 and negative catalase test).
characterization of antimicrobial susceptibility of recovered isolates to different groups of antibiotics and detection of the incidence of vancomycin resistant isolates using agar screening method were then performed. Finally, PCR was applied for detection of Enterococcus species using species-specific primers and vancomycin resistant genes among them.
Hence, one hundred and thirty five samples of milk (24), yoghurt (12), karish cheese (20), intestine (13), crop (12), chicken offals (10), chicken meat (10) and human urine (34) were analyzed. Mastitic and raw cow milk samples were collected from different dairy farms in Sharkia and Dakahlia Governorates, human urine was obtained from private laboratories and hospitals, while chicken meat and organs, cheese and yoghurt were collected randomly from different supermarkets in Sharkia and Dakahlia Governorates to be examined bacteriologically for detection of Enterococcus species.