الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
 |  | from | 32 |  |  |
| | | from | 32 | | |
Abstract
In this study a total of 117 samples were collected from individual cases of clinically diseased rabbits obtained to Animal Health Research Institute in Kafr El-sheikh for examination , in order to isolate and molecular identify of Pasteurella multocida as a causative agent of one of the most important respiratory disease in rabbits called snuffles The samples recorded as 68 samples from nasopharyngeal swabs , 43 samples from lung tissues , 2 samples from subcutaneous abscesses swabs , 3 samples from liver swabs and 1 swab from the conjunctiva with percentage of (58.12%, 36.752%, 1.71%, 2.564%, 0.854%) respectively Out of 117 samples examined, 11 isolates of Pasteurella multocida were recovered with total percentage of (9.4 % The isolates were identified on the basis of traditional phenotypic procedures as colonial morphology, microscopically examination and biochemical reactions Biochemically, Pasteurella multocida isolates were indole, catalase and oxidases positive while urea hydrolysis, citrate utilization, hydrogen sulphide production tests were negative. They failed to grow on MacConkey’s agar and not produce hemolysis on blood agar. Recently, a genotypic method of bacterial identification have proved to overcome limitations of traditional phenotypic method procedures In this study, the PCR assay technique show sensitive and accurate method for Pasteurella multocida identification. Six examined Pasteurella multocida isolates by PCR gave a single amplified product of the expected size of 460 bp Multiplex PCR assay has been successfully used in this study for detection of Pasteurella multocida virulence factors genes. The 6 examined Pasteurella multocida isolates gave a single amplified product of the expected size of (1044 bp ,488 bp and 275 bp) for capsular, type IV fimbria and hemagglutinin which are virulence factors genes for Pasteurella multocida as all of the examined samples contain capsular gene (100%) , 5/6 samples (83.3%) contain hemagglutinine and 2/6 samples (33.3%) contain type IV fimbria gene Based upon its specifity and sensitivity, the PCR assay was estimated to be a valuable and effective method for molecular detection of Pasteurella multocida isolated from rabbits.