الفهرس 
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SchistosomiasisOnly 14 pages are availabe for public view
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Abstract
Hepatocyte transplantation is an attractive therapeutic modality for liver disease as an alternative for orthotropic liver transplantation. The goal of this work was to study the adequacy of intrasplenic hepatocyte transplantation (HCTx) in fresh and microencapsulated forms, in a hamster model of liver fibrosis by Schistosoma mansoni infected hamsters and other model of liver cirrhosis by toxic dose of CCl4. The Schistosoma mansoni infected hamsters were divided into 7 groups; normal control (GI),untreated for 11 weeks (GII) and for 15 weeks (GIII), treated with praziquantel (PZQ) 7 weeks PI, and killed 4 weeks (GIV) and 8 weeks (GV) post-treatment. Treated with PZQ 7 weeks PI, and then treated orally with immunosuppressive drug ”cyclosporine (4weeks post PZQ treatment), 24 hr. before interasplenic injection with fresh hepatocytes (GVI).Treated with PZQ 7 weeks PI, and then injected intrasplenically (4 weeks post-treatment) with microencapsulated hepatocytes (GVII). The CCl4 group subdivided into four groups each has ten hamsters normal control uninjected (GVIII), negative control injected with CCl4 toxic dose intraperitoneally (i.p) for 24 hrs (GIX), injected with CCl4 toxic dose (i.p) for 24 hours, treated with cyclosporin as immuno-suppressive drug 24 hrs. post injection, then transplanted with fresh hepatocytes intrasplenically(GX) and injected with CCl4 toxic dose (i.p) for 24 hours, then transplanted with modified hepatocytes encapsulated in sodium alginate intrasplenically(GXI). Freshly isolated hepatocytes with a mean viability 92.97% 1.2% were used for microencapsulation and transplantation. Histological study showed the presence of transplanted hepatocytes in spleen of recipient. In addition, there were significantly better outcomes in serum biochemical indexes such as ALT, AST, γ-GT, ALP, and hepatic SOD and MDA in the fresh and microencapsulated groups than in PZQ-treated group, without great differences between the microencapsulate and the fresh transplanted groups. Liver pathological staining supported these findings.