الفهرس 
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Abstract
This present work was to study the diversity of certain virulent factors (coagulase and haemolysin), virulent genes and sensitivity to antimicrobial agents of Staphylococcus aureus isolates recovered from different sources. The results showed that: Bacteriological examination of 14 human samples ,74 cattle samples,46poultry samples and 10 rabbit samples yielded 12, 50, 29 and 6 isolates respectively. forty-two isolates out of the 97 suspected isolates were positive for coagulase activity. S. aureus was tested for invitro differentiation between human origin and animal origin strains using different types of blood agar medium (sheep, horse, rabbit and human). The result showed that 17 isolates (40.47%) of human origin and 25 isolates (59.52) of animal origin. Result of antimicrobial sensitivity test showed that the highest frequency of resistance was observed with Kanamycin and The least was observed with Cephalotin. PCR was used to detect S. aureus virulent genes (clfB – clfA –cna -bbp). clfB was detected in all strains(100%) in 15 isolates (4 human-6 cattle-3 poultry-2 rabbit) , 13 (86.66%) isolates(4human-5cattle-3poultry-1rabbit) of the strains were positive for clfA,7 (46.66%) isolates(3human-2cattle-1poultry-1rabbit) were positive for cna and 5 (33.33%) isolates(2human-2cattle-1poultry)were positive for bbp. Yet, that from rabbit were negative to bbp.