الفهرس 
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Abstract
Lignocellulosic biomass is the most plentiful of all the
naturally occurring organic compounds. Conversion of cellulosic
polymers into useful products by fermentation involves two stages:
firstly conversion of cellulose to glucose by cellulase, and secondly
microbial conversion of the resulting glucose to products.
The related activities toward cellubiose and β-D-glucosidase
depend on the source of the enzyme especially for A. niger. The
fascinating aspect of cellobiase produced by A.niger is its higher
yield, which is about 46 times that of Trichoderma.
The experiments were conducted to obtain β- glucosidase
and cellobiase enzyme from the two fungi strains Aspergillus
nigerNRRL3 and Aspergillus brasiliensis by using rice straw as
main component in fermentation media. The experiments were
carried out in the laboratory of Soil, Water and Environmental
Instituted, Giza Egypt. To achieve this target the following
procedures will adopted: Several agricultural wastes especially rice
straw were used as a raw material to isolate microorganisms with
capabilities to decompose the raw material cellulose. The isolated
microorganisms were purified and screened for the cellulose
degradation and for the production of the β- glucosidase enzyme.
Two fungal strains were selected that showed strong cellulose
degradation and high β- glucosidase enzyme production. The two
fungal strains were identified to species level. Nutritional and
environmental condition for the β- glucosidase enzyme production
by the two fungal strains was optimized. The β- glucosidase enzyme
was precipitated and purified.