الفهرس 
• IntroductionOnly 14 pages are availabe for public view
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Abstract
Stem cell therapy holds great promise for the repair of injured tissues and organs, including the liver. The purpose of the present study was to demonstrate the effect of transplanted mononuclear and differentiated stem cells on liver functions in experimental rats with ALF.
• The present study aimed to evaluate the feasibility of bone marrow mononuclear cells (BMMCs) to attenuate or improve recovery of induced acute liver failure animal models.
• The rats were classified into three groups:
1st group was the control group (acute liver failure-non-treated group) in which rats were undergone acute liver failure by single I.P injection of CCL4 in a dose of 3.0 ml/kg.
2nd group was BMMCs treated group in which acute liver failure rats were treated with single intravenous injection of BMMCs in a dose of 10 x 106 cells/rat.
3rd group was BMMCs derived hepatocyte-like cells treated group in which acute liver failure rats were treated with single intravenous injection of differentiated stem cells.
• Acute liver failure was induced by a single intraperitoneal (I.P.) injection of carbon tetrachloride (3.0 ml/kg dissolved in vegetable oil).
• 8-10 ml BM was aseptically collected, and under complete aseptic conditions was separated. The layer of BMMCs was collected, some of them were infused and the others were subjected to differentiation into hepatocyte- like cells and then infused.
Summery
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• Separated human BMMCs were detected by flowcytometry with monoclonal antibody specific for CD 45, CD 34 and CD 14.
• Transaminases and survival rate were measured at 72 hours, 1, 2, 3, 4 weeks after injection of either BMMCs or hepatocyte-like cells.
• Also, BMMCs in blood of rats were detected by flowcytometry with monoclonal antibody specific for CD 45, CD 34 and CD 14 after 4 weeks.
• The rats were sacrificed and livers were excised for histopathological and immunohistochemical studies.
• Single intravenous injection of either BMMCs or hepatocyte-like cells was associated with significant decrease in the mean value of serum ALT and AST and significant increase in the mean value of survival rate compared with the corresponding values in non treated group, indicating that liver deterioration can be improved by transplanted BMMCs.
• The peripheral blood of treated rats show positivity for human CD 45, CD 34 and CD14 indicating homing of BMMCs.
• Histopathological examination revealed significant decrease in the degree of necrosis, steatosis and inflammation with significant increase in the mitotic index of treated groups than non treated group, indicating that liver was already in its regenerative process.
• Immunohistochemical stained section from both treated groups showed significant positivity for both anti-human albumin and PCNA which indicated the presence of functional human cells in rat livers and proliferation of liver cells.