الفهرس 
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Abstract
Coccidiosis is a dangerous and common disease of poultry caused by several species of Eimeria protozoan-type parasites (coccidia). These parasites are generally host-specific which infect various sites of epithelial cells in the intestinal mucosa, multiply in the intestinal tract and cause tissue damage of the small intestine. The resulting damage interferes with food digestion and nutrient absorption, Dehydration and blood loss .Also, the tissue damage may lead to the affected bird be susceptible to infection by bacteria, such as Clostridia and Salmonella.
The infected stage- sporulated oocyst, is ingested and by the action of mechanical and chemical factor in the gut like Gastric Juice which contain bile salts and trypsin digest the sporulated oocyst wall and sporozoites released to invade in the epithelial cells of the intestinal mucosa.
Misuses of anticoccidial drugs to prevent or treat the chicken coccidisis lead to the development of resistance to available anticoccidial drugs and public concern over residues in meat and eggs are likely to result in increased use of live vaccines for the control of the disease because immunological control is recognized as the only practical alternative to anticoccidial drugs.
In the present study Experiment ( I ) was carried out for Propagation and Preparation of pure cecal oocysts stock from local cecal oocysts isolate.
• Oocysts are collected directly from the 2 cecii of scarified infected birds in various localities in Assiut .
• Microscopic examination of cecal scraping to detect non sporulated oocysts, Prepared clean stock of sporulated cecal oocysts (Fig. 9,10).
• Detect symptoms of coccidiosis in chickens artificially infected with sporulated oocysts suspension & macroscopic examination of post-mortum lesions in Cecum which enlarged, hemorrhagic, dilated and presence of clotted blood core of its contents (Fig. 7, 8).
• Preparation of pure cecal oocysts stock from the dropping of the infected living birds and counted by haemocytometer technique.
Part from stock used to prepare Eimeria antigen (sporozoites) after sonicated by (SONIPREP 150/MSE) at 20 KHz for 5 min in a jacketed vessel at 4-8 C. for ElISA test and Modified Splenic cell migration inhibition assay. Fig. (11)
Other part from stock used in experiment IV.
Experiment II was carried out for detection of production and releases the Bile salts from liver.
o Hay’s test to detect Bile salts in duodenal contents (Fig. 12).
o Confirm on the bile start to present in duodenal contents at 4th day old age of chick.
Experiment III was carried out for detection of production and releases the Trypsin from Pancreas.
o Semiquentitative determination of Trypsin by X-ray film test (Fig. 13).
o Confirm on the trypsin start to present in duodenal contents at 4th day old age of chick and reach to maximum concentration at 6th day of chick age.
Experiment IV was carried out for detect the immunological response to anticoccidial vaccine when used in different ages.
o Test was conducted to assess whether the age at which birds were initially exposed to coccidian vaccine would affect their ability to develop absolute immunity.
o In this experiment 1st day, 4th day and age when confirm the production of bile salts and trypin at 6th day of age according to the experiment II & III.
o By using Elisa test to detect the Humoral immunity and Splenic cell migration index test to detect the Cellular immunity.
o The best age of chick for vaccination is 6th days of age according to results in (Tab. 4 : 10) (Fig. 24 : 31).
Experiment V was carried out for detect the immunological response to anticoccidial vaccine when used in different methods of administration.
o Test was conducted to assess whether the mode of administration at which birds were vaccinated (food or eye drop) would affect their ability to develop absolute immunity.
o Each group of the 1 day, 4 day & 6 days of age will divided to 2 groups one group vaccinated by food intake and other group vaccinated by eye DROP method.
o By using Elisa test to detect the Humoral immunity and Splenic cell migration index test to detect the Cellular immunity.
o The best mode of administration for vaccination is eye DROP method according to results in (Tab.4 : 10 ) (Fig. 24 : 31 ).
Experiment VI was carried out for assess the efficacy of anticoccidial drugs in treated group incomparable with Vaccinated group in experimental infected broiler chickens with sporulated Cecal coccidian oocysts.
o The evaluation based on calculate the mortality (Survival percent), weight gain or loss (percent ratio of mean gain), fecal score, lesion score and Performance index.
o The vaccinated group is better than usage of anticoccidial medication in all evaluated items with high significant difference over treated medicated group ( Tab.1 ) (Fig 16:22 ).
o The performance index of the infected vaccinated group is higher than in infected medicated group with significant difference (Tab .2) (Fig. 23).
o Finally the usage of anticoccidial vaccine is better than usage of anticoccidial medication.